A PSEUDOMONAS-PUTIDA STRAIN ABLE TO DEGRADE M-TOLUATE IN THE PRESENCE OF 3-CHLOROCATECHOL

被引：28

作者：

WASSERFALLEN, A ^{[1
]}

REKIK, M ^{[1
]}

HARAYAMA, S ^{[1
]}

机构：

[1] UNIV GENEVA,MED CTR,DEPT MED BIOCHEM,1 RUE MICHEL SERVET,CH-1211 GENEVA 4,SWITZERLAND

来源：

BIO-TECHNOLOGY | 1991年 / 9卷 / 03期

关键词：

D O I：

10.1038/nbt0391-296

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Growth on m-toluate of Pseudomonas putida carrying the TOL plasmid is inhibited by 3-chlorocatechol. From a P. putida strain, carrying cloned TOL genes responsible for the oxidation of methylbenzoates, we isolated a derivative able to grow on m-toluate in the presence of 3-chlorobenzoate. A point mutation in xylE, the structural gene for catechol 2,3-dioxygenase, which produced the substitution of valine-291 for isoleucine-291, was responsible for the growth characteristics of the isolate. The mutant catechol 2,3-dioxygenase has (i) decreased affinity for 3-chlorocatechol, (ii) increased affinity for 3-methylcatechol, and (iii) increased efficiency in oxidizing 4-ethylcatechol, compared to the wild-type enzyme. This strain may be useful for the decomposition of m-toluate in industrial waste containing both 3-chlorobenzoate and m-toluate.

引用

页码：296 / 298

页数：3

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