96-WELL APPARATUS AND METHOD FOR USE IN ENZYME-LINKED IMMUNOFILTRATION ASSAY (ELIFA)

被引：13

作者：

CLARK, CR

HINES, KK

MALLIA, AK

机构：

[1] Research and Development, Pierce Chemical Company, Rockford, 61105, IL

来源：

BIOTECHNOLOGY TECHNIQUES | 1993年 / 7卷 / 06期

关键词：

D O I：

10.1007/BF00155481

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

An apparatus and method are described for use in enzyme-linked immunofiltration assay (ELIFA). The method of filtering ligands, blockers, wash buffers, and substrates decreases a typical 30 min to 2 hr incubation step with ELISA to 5 minutes. The apparatus differs from a dot-blot apparatus in that soluble coloured product is transferred directly into a receiving 96-well microtiter plate for quantifying with an automatic plate reader. Model systems of streptavidin/biotin and antibody/antigen form detectable complexes in the apparatus.

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页码：461 / 466

页数：6

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