EXPRESSION OF FRAGMENT C OF TETANUS TOXIN FUSED TO A CARBOXYL-TERMINAL FRAGMENT OF DIPHTHERIA-TOXIN IN SALMONELLA-TYPHI CVD-908 VACCINE STRAIN

We report the expression of fragment C of tetanus toxin (FC) fused to the eukaryotic cell binding domain (the carboxyl-terminus) of diphtherin toxin (FC-bDt fusion) in attenuated Salmonella typhi live vector vaccine strain CVD 908, The FC-bDt protein fusion was constructed using plasmid p TET nir 15 which carries the gene encoding FC under control of the nirB promoter (P-nirB). The open reading frame for FC was modified to incorporate an in-frame glycine-proline hinge region and a set of four restriction sites at the 3' end of the FC gene. A 482 bp DNA fragment encoding the eukaryotic cell binding domain of diphtheria toxin was then inserted at the 3' end of the modified FC gene to create an in-frame FC-bDt fusion gene. The resulting plasmid, pOG215, was able to express the FC-bDt fusion protein in both Escherichia coli DH5a and S. typhi CVD 908, as evidenced by Western immunoblots using anti-FC and anti-C-terminal diphtheria toxin monoclonal antibodies. Maximum expression of the FC-bDt fusion protein was achieved by growing CVD 908(pOG215) at the low oxidation-reduction potential of thioglycollate broth, i.e. in conditions that activate P-nirB and drive transcription of the FC-bDt fusion gene, Whereas maximum expression of FC alone was also observed using thioglycollate broth, expression of bDt alone was unsuccessful using a variety of growth conditions. FC fusions constitute one strategy to ''rescue'' expression of proteins, which are otherwise difficult to express.