DECARBOXYLATION OF UROPORPHYRINOGEN-III BY ERYTHROCYTE UROPORPHYRINOGEN DECARBOXYLASE - EVIDENCE FOR A RANDOM DECARBOXYLATION MECHANISM

The isomeric composition of type-III heptacarboxylic porphyrinogens derived from decarboxylation of uroporphyrinogen III by erythrocyte uroporphyrinogen decarboxylase was analysed by h.p.l.c. with electrochemical detection. All four possible isomers were identified, and there were little differences in the proportion of isomers formed by erythrocytes from normal subjects and from patients with sporadic porphyria cutanea tarda. The results provide conclusive evidence that the normal decarboxylation pathway is random in nature, and the fourth isomer only increases when enzyme abnormality is found.