IMAGING THE REJECTING HEART - INVIVO DETECTION OF MAJOR HISTOCOMPATIBILITY COMPLEX CLASS-II ANTIGEN INDUCTION

被引:33
作者
ISOBE, M
NARULA, J
SOUTHERN, JF
STRAUSS, HW
KHAW, BA
HABER, E
机构
[1] MASSACHUSETTS GEN HOSP, DEPT RADIOL, DIV NUCL MED, BOSTON, MA 02114 USA
[2] HARVARD UNIV, SCH MED, BOSTON, MA 02115 USA
关键词
TRANSPLANTATION; SCINTIGRAPHY; CYCLOSPORINE; MHC REJECTION;
D O I
10.1161/01.CIR.85.2.738
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Background. Mice with abdominal heterotopic heart transplants were studied to determine whether scintigraphic detection of an increase in major histocompatibility complex (MHC) class II antigen expression could be used as a noninvasive method for diagnosing early rejection. Methods and Results. Allografts from C3H/He (H2k) donors were transplanted into BALB/c (H2(d)) recipients (n = 18). Two of the 18 allografted mice were treated with cyclosporine (15 mg/kg/day), and two isografted mice served as controls. Each mouse was injected intravenously with 100-mu-Ci of In-111-labeled anti-MHC class II monoclonal antibodies (10-2-16 and 14-4-4S) 24 hours before scintigraphy. After imaging, the mice were killed for tissue counting and histopathology. Radiotracer uptake in the grafts reflected the severity of rejection as determined by histopathological criteria. The percent injected dose per gram of tissue in excised grafts was 4.8 +/- 1.8 (mean +/- SD) for normal grafts (n = 8), 11.1 +/- 9.7 for grafts with grade IA rejection (n = 3, NS), 18.0 +/- 3.8 for grafts with grade IIIA rejection (n = 4, p < 0.001 versus normal), 18.7 +/- 3.2 for grafts with grade IIIB rejection (n = 3, p < 0.001 versus normal), and 22.6 +/- 5.4 for grafts with severe rejection (grade IV) (n = 3, p < 0.001 versus normal). Rejecting allografts with lymphocyte infiltration but without significant myocyte necrosis could be identified by this scintigraphic method. In the BALB/c donor-C57BL/6 (H2b, IE-) recipient combination, rejecting allografts were visualized by 14-4-4S (anti-IE(k,d,p,r)) antibody but not by 10-2-16 (anti-IA(k,r,s,f) antibody. This difference shows that class II antigens induced on donor hearts are solely responsible for the antibody uptake in positive scintigrams of rejecting allografts. Conclusions. We conclude that In-111-labeled anti-MHC class II antigen antibody imaging is a sensitive and noninvasive method for detecting cardiac allograft rejection.
引用
收藏
页码:738 / 746
页数:9
相关论文
共 42 条
[1]  
ABB J, 1983, TRANSPLANT P, V15, P2380
[2]   IMAGING OF CARDIAC ALLOGRAFT-REJECTION IN DOGS USING IN-111 MONOCLONAL ANTIMYOSIN FAB [J].
ADDONIZIO, LJ ;
MICHLER, RE ;
MARBOE, C ;
ESSER, PE ;
JOHNSON, LL ;
SELDIN, DW ;
GERSONY, WM ;
ALDERSON, PO ;
ROSE, EA ;
CANNON, PJ .
JOURNAL OF THE AMERICAN COLLEGE OF CARDIOLOGY, 1987, 9 (03) :555-564
[3]   MAJOR HISTOCOMPATIBILITY COMPLEX CLASS-I AND CLASS-II EXPRESSION BY MYOCYTES IN CARDIAC BIOPSIES POSTTRANSPLANTATION [J].
AHMEDANSARI, A ;
TADROS, TS ;
KNOPF, WD ;
MURPHY, DA ;
HERTZLER, G ;
FEIGHAN, J ;
LEATHERBURY, A ;
SELL, KW .
TRANSPLANTATION, 1988, 45 (05) :972-978
[4]   DETECTION OF CARDIAC ALLOGRAFT-REJECTION AND MYOCYTE NECROSIS BY MONOCLONAL-ANTIBODY TO CARDIAC MYOSIN [J].
ALLEN, MD ;
TSUBOI, H ;
TOGO, T ;
EARY, JF ;
GORDON, D ;
THOMAS, R ;
REICHENBACH, DD .
TRANSPLANTATION, 1989, 48 (06) :923-928
[5]  
APPLEYARD ST, 1985, LANCET, V1, P361
[6]   INDIUM-111-MONOCLONAL ANTIMYOSIN ANTIBODY STUDIES AFTER THE 1ST YEAR OF HEART-TRANSPLANTATION - IDENTIFICATION OF RISK GROUPS FOR DEVELOPING REJECTION DURING LONG-TERM FOLLOW-UP AND CLINICAL IMPLICATIONS [J].
BALLESTER, M ;
OBRADOR, D ;
CARRIO, I ;
AUGE, JM ;
MOYA, C ;
PONSLLADO, G ;
CARALPSRIERA, JM .
CIRCULATION, 1990, 82 (06) :2100-2108
[7]  
BENSON EM, 1985, J IMMUNOL, V134, P7
[8]  
Billingham M E, 1990, J Heart Transplant, V9, P587
[9]   CORRELATION BETWEEN CLASS II ANTIGEN (DR) EXPRESSION AND INTERLEUKIN-2-INDUCED LYMPHOCYTE-PROLIFERATION DURING ACUTE CARDIAC ALLOGRAFT-REJECTION [J].
CARLQUIST, JF ;
HAMMOND, ME ;
YOWELL, RL ;
OCONNELL, JB ;
ANDERSON, JL .
TRANSPLANTATION, 1990, 50 (04) :582-588
[10]   THE DETAILED DISTRIBUTION OF HLA-A-ANTIGEN, B-ANTIGEN, C-ANTIGEN IN NORMAL HUMAN ORGANS [J].
DAAR, AS ;
FUGGLE, SV ;
FABRE, JW ;
TING, A ;
MORRIS, PJ .
TRANSPLANTATION, 1984, 38 (03) :287-292