PYRIDINYL IMIDAZOLES INHIBIT IL-1 AND TNF PRODUCTION AT THE PROTEIN LEVEL

被引：114

作者：

YOUNG, P ^{[1
]}

MCDONNELL, P ^{[1
]}

DUNNINGTON, D ^{[1
]}

HAND, A ^{[1
]}

LAYDON, J ^{[1
]}

LEE, J ^{[1
]}

机构：

[1] SMITHKLINE BEECHAM PHARMACEUT,DEPT CELLULAR BIOCHEM,KING OF PRUSSIA,PA 19406

来源：

AGENTS AND ACTIONS | 1993年 / 39卷

关键词：

D O I：

10.1007/BF01972723

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The mechanism by which SK&F 86002 and other pyridinyl imidazoles inhibit the production of IL-1 and TNF from LPS-stimulated human monocytes was examined. Inhibition of IL-1 and TNF production was found to depend on the time of addition of SK&F 86002, with diminishing effect when added more than 2 h after LPS stimulation. Analysis of Western blots confirmed that both intracellular IL-1beta and extracellular TNF were significantly reduced in response to SK&F 86002, but these reductions were not paralleled by changes in IL-1 and TNF mRNA. S-35 methionine pulse and pulse-chase studies on IL-1 biosynthesis suggest that significant inhibition by SK&F 86002 and related compounds occurs at the translational level.

引用

页码：C67 / C69

页数：3

共 8 条

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