ABERRANT GAG PROTEIN-COMPOSITION OF A HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 VIF MUTANT - PRODUCED IN PRIMARY LYMPHOCYTES

被引:106
作者
SIMM, M
SHAHABUDDIN, M
CHAO, W
ALLAN, JS
VOLSKY, DJ
机构
[1] ST LUKES ROOSEVELT HOSP, MOLEC VIROL LAB, NEW YORK, NY 10019 USA
[2] COLUMBIA UNIV, COLL PHYS & SURG, NEW YORK, NY 10019 USA
[3] SW FDN BIOMED RES, DEPT VIROL & IMMUNOL, SAN ANTONIO, TX USA
关键词
D O I
10.1128/JVI.69.7.4582-4586.1995
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Productive, spreading infection of peripheral blood lymphocytes (PBL) with human immunodeficiency virus type 1 (HIV-1) requires the viral protein Vif. To study the requirement for vif in this system, we infected PBL with a phenotypically complemented HIV-1 clone mutated in vif. Progeny virus was produced which was noninfectious in PBL but replicated in SupT1 cells. Analysis of metabolically labeled proteins of sedimentable extracellular particles made in PBL by radioimmunoprecipitation with either serum from a patient with AIDS or a monoclonal antibody reactive with HIV-1 Gag proteins revealed that vif-negative but not wild-type particles carry higher levels of p55, p41, and p38 Gag-specific proteins compared with those of p24. Similar results were obtained with sucrose-purified virions. Our data indicate that vif plays a role in Gag protein processing or in incorporation of processed Gag products into mature virions. The presence of unprocessed precursor Gag polyprotein (Pr55(gag)) and other Gag processing intermediates in PBL-derived vif-negative extracellular particles may contribute to the reduced infectivity of this virus.
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页码:4582 / 4586
页数:5
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