CLONING OF ALGINATE LYASE GENE (ALXM) AND EXPRESSION IN ESCHERICHIA-COLI

被引：14

作者：

BROWN, BJ ^{[1
]}

PRESTON, JF ^{[1
]}

INGRAM, LO ^{[1
]}

机构：

[1] UNIV FLORIDA,MICROBIOL & CELL SCI,GAINESVILLE,FL 32611

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 1991年 / 57卷 / 06期

关键词：

D O I：

10.1128/AEM.57.6.1870-1872.1991

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The alxM gene encoding a D-mannuronan-specific alginate lyase has been cloned from a marine bacterium isolated as an epiphyte on the brown alga, Sargassum fluitans. Expression of this gene in Escherichia coli provides a source of this enzyme for probing alginate structure and modifying the mannuronan-rich alginate polymers produced by bacterial pathogens.

引用

页码：1870 / 1872

页数：3

共 25 条

[1]

BAARDSETH E, 1966, 5TH P INT SEAW S, P19

[2] STRUCTURAL STUDIES OF ALGINIC ACID, USING A BACTERIAL POLY-ALPHA-L-GULURONATE LYASE [J].

BOYD, J ;

TURVEY, JR .

CARBOHYDRATE RESEARCH, 1978, 66 (OCT) :187-194

[3] STUDIES OF ALGINATE DEGRADING ENZYMES .1. ISOLATION OF A POLY-ALPHA-L-GULURONATE LYASE FROM KLEBSIELLA-AEROGENES [J].

BOYD, J ;

TURVEY, JR .

CARBOHYDRATE RESEARCH, 1977, 57 (AUG) :163-171

[4] CONSTRUCTION OF A NEW VECTOR FOR THE EXPRESSION OF FOREIGN GENES IN ZYMOMONAS-MOBILIS [J].

BYUN, MOK ;

KAPER, JB ;

INGRAM, LO .

JOURNAL OF INDUSTRIAL MICROBIOLOGY, 1986, 1 (01) :9-15

[5] MOLECULAR-CLONING AND HETEROLOGOUS EXPRESSION OF A KLEBSIELLA-PNEUMONIAE GENE ENCODING ALGINATE LYASE [J].

CASWELL, RC ;

GACESA, P ;

LUTRELL, KE ;

WEIGHTMAN, AJ .

GENE, 1989, 75 (01) :127-134

[6] CLONING OF PSEUDOMONAS-AERUGINOSA ALGG, WHICH CONTROLS ALGINATE STRUCTURE [J].

CHITNIS, CE ;

OHMAN, DE .

JOURNAL OF BACTERIOLOGY, 1990, 172 (06) :2894-2900

[7] PROMOTER AND NUCLEOTIDE-SEQUENCES OF THE ZYMOMONAS-MOBILIS PYRUVATE DECARBOXYLASE [J].

CONWAY, T ;

OSMAN, YA ;

KONNAN, JI ;

HOFFMANN, EM ;

INGRAM, LO .

JOURNAL OF BACTERIOLOGY, 1987, 169 (03) :949-954

[8] AN ENZYMIC METHOD FOR THE ASSAY OF D-MANNURONAN C-5 EPIMERASE ACTIVITY [J].

CURRIE, AJ ;

TURVEY, JR .

CARBOHYDRATE RESEARCH, 1982, 107 (01) :156-159

[9]

DOUBET RS, 1984, APPL ENVIRON MICROB, V47, P600

[10] PARTIAL-PURIFICATION AND CHARACTERIZATION OF A POLYMANNURONIC ACID DEPOLYMERASE PRODUCED BY A MUCOID STRAIN OF PSEUDOMONAS-AERUGINOSA ISOLATED FROM A PATIENT WITH CYSTIC-FIBROSIS [J].

DUNNE, WM ;

BUCKMIRE, FLA .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1985, 50 (03) :562-567

← 1 2 3 →