NERVE GROWTH-FACTOR BIOSYNTHESIS - ISOLATION AND CHARACTERIZATION OF A GUINEA-PIG PROSTATE KALLIKREIN

被引：14

作者：

DUNBAR, JC

BRADSHAW, RA

机构：

来源：

JOURNAL OF CELLULAR BIOCHEMISTRY | 1985年 / 29卷 / 04期

关键词：

D O I：

10.1002/jcb.240290405

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Guinea pig prostate contains one major soluble esteropeptidase activity. The protein has been purified and characterized and found to be a glycoprotein comprised of a single polypeptide chain. The molecular weight of the deglycosylated protein is approximately 26,000. The esteropeptidase has a similar Km for lysine and argine synthetic substrates, although the Vmax for arginine is much greater than that for lysine. Amino-terminal sequence analysis has also revealed a marked degree of homology of mouse .gamma.-nerve growth factor (NGF) and the kallikrein family of serine proteases. In contrast to .gamma.-NGF, however, the guineapig enzyme does not appear to form stable complexes with .beta.-NGF.

引用

页码：309 / 319

页数：11

共 24 条

[1] NERVE GROWTH FACTOR FROM MOUSE SUBMAXILLARY GLAND - AMINO ACID SEQUENCE
ANGELETT.RH
BRADSHAW, RA
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1971, 68 (10) : 2417 - &
[2] BERGER EA, 1978, J BIOL CHEM, V253, P804
[3] BOTHWELL MA, 1979, J BIOL CHEM, V254, P7287
[4] Colman R W, 1976, Methods Enzymol, V45, P303
[5] DEGLYCOSYLATION OF GLYCOPROTEINS BY TRIFLUOROMETHANESULFONIC ACID
EDGE, ASB
FALTYNEK, CR
HOF, L
REICHERT, LE
WEBER, P
[J]. ANALYTICAL BIOCHEMISTRY, 1981, 118 (01) : 131 - 137
[6] FIELDER F, 1979, HDB EXPT PHARM S, V25, P103
[7] SUBUNIT INTERACTION AND ENZYMATIC ACTIVITY OF MOUSE 7S NERVE GROWTH FACTOR
GREENE, LA
SHOOTER, EM
VARON, S
[J]. BIOCHEMISTRY, 1969, 8 (09) : 3735 - +
[8] GUINEA-PIG PROSTATE IS A RICH SOURCE OF NERVE GROWTH-FACTOR
HARPER, GP
BARDE, YA
BURNSTOCK, G
CARSTAIRS, JR
DENNISON, ME
SUDA, K
VERNON, CA
[J]. NATURE, 1979, 279 (5709) : 160 - 162
[9] MOUSE 7S NERVE GROWTH-FACTOR - COMPLETE SEQUENCE OF A CDNA CODING FOR THE ALPHA-SUBUNIT PRECURSOR AND ITS RELATIONSHIP TO SERINE PROTEASES
ISACKSON, PJ
ULLRICH, A
BRADSHAW, RA
[J]. BIOCHEMISTRY, 1984, 23 (25) : 5997 - 6002
[10] MOUSE NERVE GROWTH-FACTOR - RAPID ISOLATION PROCEDURE FOR THE ALPHA AND GAMMA SUBUNITS
JENG, I
ANDRES, RY
BRADSHAW, RA
[J]. ANALYTICAL BIOCHEMISTRY, 1979, 92 (02) : 482 - 488

← 1 2 3 →