DETECTION AND IDENTIFICATION OF ENDOTHELIN-1 IMMUNOREACTIVITY IN RAT AND PORCINE THYROID FOLLICULAR CELLS

被引：39

作者：

COLIN, I ^{[1
]}

BERBINSCHI, A ^{[1
]}

DENEF, JF ^{[1
]}

KETELSLEGERS, JM ^{[1
]}

机构：

[1] CATHOLIC UNIV LOUVAIN,SCH MED,UNITE HISTOL,B-1200 BRUSSELS,BELGIUM

来源：

ENDOCRINOLOGY | 1992年 / 130卷 / 01期

关键词：

D O I：

10.1210/en.130.1.544

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Endothelin-1 immunoreactivity (irET-1) was observed in rat and porcine thyroid glands. Using a radioimmunoassay for endothelin-1, the mean concentration in extracts of rat and porcine thyroid glands were 0.75 pg/mg +/- 0.03 (n = 4) and 1.5 pg/mg +/- 0.2 (n = 8) (mean +/- SE) respectively. Gel-filtration and reverse-phase HPLC showed that ir ET-1 eluted in a position identical to synthetic endothelin-1. In addition, immunohistochemical study showed that irET-1 is located within epithelial follicular cells. No immunostaining was seen in parafollicular C-cells nor in parathyroid.

引用

页码：544 / 546

页数：3

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