OPENING OF THE BLOOD-BRAIN-BARRIER DURING CORTICAL SUPERFUSION WITH HISTAMINE

被引:50
作者
SCHILLING, L
WAHL, M
机构
[1] Department of Physiology, Ludwig-Maximilians University, D-80336 Munich
关键词
EXTRAVASATION; BLOOD-BRAIN BARRIER; HISTAMINE; IMPROMIDINE; 2-PYRIDYLETHYLAMINE; INTRAVITAL FLUORESCENCE MICROSCOPY;
D O I
10.1016/0006-8993(94)90403-0
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Histamine may influence cerebral microcirculation from the intravascular and parenchymal side. The latter route can be simulated by cortical superfusion. The effect of cortical superfusion with histamine (10(-9)-10(-3) M) on blood-brain barrier (BBB) permeability was studied in the cat by measuring extravasation of the tracers Na+-fluorescein (MW 376) or fluorescein isothiocyanate (FITC) labelled dextran (MW 62,000 or 145,000) by intravital fluorescence microscopy. Histamine induced an opening of BBB resulting in extravasation of small and large molecular weight tracers with threshold concentrations of 10(-9) 10(-8) and 10(-6) M for Na+-fluorescein, FITC-dextran 62,000 and 145,000, respectively. Once tracer extravasation had started the degree of extravasation increased with increasing concentrations of histamine in the superfusion fluid. Similar to histamine the H-2 agonist impromidine (3 x 10(-12)-3 x 10(-9) M) induced a concentration dependent extravasation of Nai-fluorescein, 2-Pyridylethylamine which is 3-4 times more selective for H-1 than for H-2 receptors also induced an extravasation of Na+-fluorescein. Cortical superfusion with mepyramine (10(-7) M) or cimetidine (10(-4) M), which block the H-1 and H-2 receptors, respectively, already induced significant extravasation of Na+-fluorescein by themselves. These compounds could thus not be used as competitive antagonists to block histamine-induced extravasation. However, our data are in accord with data obtained during intravascular and topical application of histamine and support the hypothesis that H-2 receptors at the luminal and abluminal membrane of the endothelium mediate the opening of the BBB. In addition, histamine induced an increase of pial venular and arteriolar diameter in concentrations above 10(-8) and 10(-6) M, respectively. This may increase the driving force for extravasation. Thus, the role of histamine as a mediator of vasogenic brain edema is considered.
引用
收藏
页码:289 / 296
页数:8
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