A PUTATIVE 4TH NA+,K+-ATPASE ALPHA-SUBUNIT GENE IS EXPRESSED IN TESTIS

被引：222

作者：

SHAMRAJ, OI ^{[1
]}

LINGREL, JB ^{[1
]}

机构：

[1] UNIV CINCINNATI, COLL MED, DEPT MOLEC GENET BIOCHEM & MICROBIOL, CINCINNATI, OH 45267 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1994年 / 91卷 / 26期

关键词：

ATP1AL2; SODIUM PUMP; ALPHA-4; ISOFORM; TISSUE-SPECIFIC GENE EXPRESSION;

D O I：

10.1073/pnas.91.26.12952

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The Na+,K+-ATPase alpha subunit has three known isoforms, alpha 1, alpha 2 and alpha 3, each encoded by a separate gene. This study was undertaken to determine the functional status of a fourth human alpha-like gene, ATP1AL2. Partial genomic sequence analysis revealed regions exhibiting sequence similarity with exons 3-6 of the Na+,K+-ATPase alpha isoform genes. ATP1AL2 cDNAs spanning the coding sequence of a novel P-type ATPase alpha subunit were isolated from a rat testis library. The predicted polypeptide is 1028 amino acids long and exhibits 76-78% identity with the rat Na+,K+-ATPase alpha 1, alpha 2 and alpha 3 isoforms, indicating that ATP1AL2 may encode a fourth Na+,K+-ATPase a isoform. A 3.9-kb mRNA is expressed abundantly in human and rat testis.

引用

页码：12952 / 12956

页数：5

共 35 条

[1]

Bok D., 1982, STRUCTURE EYE, P247

[2] DISTRIBUTION OF SODIUM-POTASSIUM ATPASE IN THE RAT TESTIS AND EPIDIDYMIS [J].

BYERS, S ;

GRAHAM, R .

AMERICAN JOURNAL OF ANATOMY, 1990, 188 (01) :31-43

[3]

CHELLY J, 1989, P NATL ACAD SCI USA, V81, P1991

[4]

CHOMCZYNSKI P, 1987, ANAL BIOCHEM, V162, P156, DOI 10.1016/0003-2697(87)90021-2

[5] GENOMIC SEQUENCING [J].

CHURCH, GM ;

GILBERT, W .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1984, 81 (07) :1991-1995

[6] EVIDENCE THAT THE ALPHA-ISOFORMS AND ALPHA-(+) ISOFORMS OF THE CATALYTIC SUBUNIT OF (NA+,K+)-ATPASE RESIDE IN DISTINCT CILIARY EPITHELIAL-CELLS OF THE MAMMALIAN EYE [J].

COCAPRADOS, M ;

LOPEZBRIONES, LG .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1987, 145 (01) :460-466

[7]

CROWSON MS, 1992, J BIOL CHEM, V267, P13740

[8]

GREEB J, 1989, J BIOL CHEM, V264, P18569

[9]

HINZ HR, 1990, J BIOL CHEM, V265, P10260

[10] STRUCTURE OF THE ALPHA-1 SUBUNIT OF HORSE NA,K-ATPASE GENE [J].

KANO, I ;

NAGAI, F ;

SATOH, K ;

USHIYAMA, K ;

NAKAO, T ;

KANO, K .

FEBS LETTERS, 1989, 250 (01) :91-98

← 1 2 3 4 →