CLONING OF ESCHERICHIA-COLI PENICILLIN-G ACYLASE GENE WITH MINI-MU CONTAINING A PLASMID REPLICON

被引：4

作者：

LACZAOVA, A ^{[1
]}

PECHAN, T ^{[1
]}

STUCHLIK, S ^{[1
]}

KORMUTAKOVA, R ^{[1
]}

TURNA, J ^{[1
]}

机构：

[1] COMENIUS UNIV BRATISLAVA, DEPT MOLEC BIOL, BRATISLAVA 84215, SLOVAKIA

来源：

BIOTECHNOLOGY LETTERS | 1995年 / 17卷 / 01期

关键词：

D O I：

10.1007/BF00134188

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

An in vivo cloning system based on mini-Mu derivatives was used for cloning of E. coli penicillin G acylase gene (pac). We have constructed several recombinant clones producing penicillin G acylase and some of them exhibit approximately two times higher activity than original strains.

引用

页码：13 / 18

页数：6

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