GENETIC-ANALYSIS OF CINNAMYL ALCOHOL-DEHYDROGENASE IN LOBLOLLY-PINE - SINGLE-GENE INHERITANCE, MOLECULAR CHARACTERIZATION AND EVOLUTION

被引：71

作者：

MACKAY, JJ

LIU, WW

WHETTEN, R

SEDEROFF, RR

OMALLEY, DM

机构：

[1] N CAROLINA STATE UNIV, DEPT FORESTRY, RALEIGH, NC 27695 USA

[2] N CAROLINA STATE UNIV, DEPT GENET, RALEIGH, NC 27695 USA

[3] N CAROLINA STATE UNIV, DEPT BIOCHEM, RALEIGH, NC 27695 USA

来源：

MOLECULAR AND GENERAL GENETICS | 1995年 / 247卷 / 05期

关键词：

CINNAMYL ALCOHOL DEHYDROGENASE; PINE; LIGNIN BIOSYNTHESIS; DNA SEQUENCE; GENOME; EVOLUTION;

D O I：

10.1007/BF00290344

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The gene encoding the monolignol biosynthetic enzyme cinnamyl alcohol dehydrogenase (CAD, E.C. 1.1.1.195) can be expressed in response to different developmental and environmental cues. Control of Cad gene expression could involve either differential regulation of more than one Cad gene or, alternatively combinatorial regulation of a single Cad gene. In loblolly pine (Pinus taeda L.), we found several electrophoretic variants (allozymes) of CAD and a high level of heterozygosity (h(e) = 0.46). Analysis of inheritance patterns of pine CAD allozymes gave segregation ratios that were consistent with Mendelian expectations for a single functional gene. The identity of the full-length Cad cDNA sequence was confirmed by alignment with peptide sequences obtained from purified active enzyme and by extensive similarity to Cad sequences from other species. Southern blot analysis of genomic DNA using the Cad cDNA as a hybridization probe gave simple patterns, consistent with our interpretation that pine Cad is a single-copy gene. Phylogenetic analysis and evolution rate estimates showed that Can sequences are diverging less rapidly in the gymnosperms than in the angiosperms. The Cad mRNA was present in both lignifying tissues and a non lignifying tissue (the megagametophyte) of pine. The presence of a single gene suggests that different regulatory mechanisms for a single Cad gene, rather than differential regulation of several genes, can account for its expression in response to different cues.

引用

页码：537 / 545

页数：9

共 51 条

[1]

Ahmad I., Finkelstein J.A., Steggles A.W., The analysis of RNA by In situ agarose gel hybridization is more sensitive than the equivalent Northern blot analysis, Biotechniques, 8, pp. 162-164, (1990)

[2]

Binns A.N., Chen R.H., Wood H.N., Lynn D.G., Cell division promoting activity of naturally occurring dehydroconiferyl glucosides: Do cell wall components control cell division, Proc Natl Acad Sci USA, 84, pp. 980-984, (1987)

[3]

Bao W., O'Malley D.M., Whetten R., Sederoff R.R., A laccase associated with lignification in loblolly pine xylem, Science, 260, pp. 672-674, (1993)

[4]

Bousquet J., Strauss S.H., Doerksen A.H., Price R.A., Extensive variation in evolutionary rate of rbcL gene sequences among seed plants, Proc Natl Acad Sci USA, 89, pp. 7844-7848, (1992)

[5]

Campbell M.M., Ellis B.E., Fungal elicitor-mediated responses in pine cell cultures. III. Purification and characterization of phenylalanine ammonia-lyase, Plant Physiol, 98, pp. 62-70, (1992)

[6]

Chang S., Puryear J., Cairney J., A simple and efficient method for isolating RNA from pine trees, Plant Mol Biol Rep, 11, pp. 113-116, (1993)

[7]

Devereaux J., Haeberli P., Smithies O., A comprehensive set of sequence analysis programs for the VAX, Nucleic Acids Research, 12, pp. 387-395, (1984)

[8]

Devey M.E., Jertad K.D., Tauer C.G., Neale D.B., Inheritance of RFLP loci in a loblolly pine three-generation pedigree, Theor Appl Genet, 83, pp. 238-242, (1991)

[9]

Felsenstein J., PHYLIP (Phylogeny Inference Package) version 3.5c, (1993)

[10]

Frohman M.A., Dush M.K., Martin G.R., Rapid production of full-length cDNAs from rare transcripts: Amplification using a single gene-specific oligonucleotide primer, Proc Natl Acad Sci USA, 85, pp. 8998-9002, (1988)

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