STRUCTURE AND CHROMOSOMAL LOCALIZATION OF THE GENE (BDKRB2) ENCODING HUMAN BRADYKININ B-2 RECEPTOR

被引:92
作者
MA, JX
WANG, DZ
WARD, DC
CHEN, LM
DESSAI, T
CHAO, J
CHAO, L
机构
[1] MED UNIV S CAROLINA,DEPT BIOCHEM & MOLEC BIOL,CHARLESTON,SC 29425
[2] YALE UNIV,SCH MED,DEPT GENET,NEW HAVEN,CT 06510
关键词
D O I
10.1006/geno.1994.1512
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The bradykinin B-2 receptor (BDKRB2) has high affinity for the intact kinins, which mediate a wide spectrum of biological effects, including pain, inflammation, vasodilation, and smooth muscle contraction and relaxation. In the present study, we have cloned and sequenced the gene encoding human bradykinin B-2 receptor from a human genomic library. The B-2 receptor gene contains three exons separated by two introns. The first and second exons are noncoding, while the third exon contains the full-length coding region, which encodes a protein of 364 amino acids forming 7 transmembrane domains. The human Bp gene shares high sequence identity with rat and mouse Bp receptor genes and significant similarity with the gene encoding the angiotensin II type I receptor in the nucleotide sequence and exon-intron arrangement. In the 5' flanking region, a consensus TATA box and several putative transcription factor-binding sites have been identified. Genomic Southern blot analysis showed that the B-2 receptor is encoded by a single-copy gene that was localized to chromosome 14q32 by in situ hybridization. In a Southern blot analysis following reverse transcription and polymerase chain reaction, the human B-2 receptor was found to be expressed in most human tissues. (C) 1994 Academic Press, Inc.
引用
收藏
页码:362 / 369
页数:8
相关论文
共 29 条
  • [1] BATHON JM, 1991, ANNU REV PHARMACOL, V31, P129
  • [2] REGULATION OF PROSTAGLANDIN SYNTHESIS MEDIATED BY THROMBIN AND B2-BRADYKININ RECEPTORS IN A FIBRO-SARCOMA CELL-LINE
    BECHERER, PR
    MERTZ, LF
    BAENZIGER, NL
    [J]. CELL, 1982, 30 (01) : 243 - 251
  • [3] BHOOLA KD, 1992, PHARMACOL REV, V44, P1
  • [4] BILLINGSLEY GD, 1993, AM J HUM GENET, V52, P343
  • [5] RECENT DEVELOPMENTS IN THE UNDERSTANDING OF BRADYKININ RECEPTORS
    BURCH, RM
    KYLE, DJ
    [J]. LIFE SCIENCES, 1992, 50 (12) : 829 - 838
  • [7] BRADYKININ RECEPTOR ANTAGONISTS
    BURCH, RM
    FARMER, SG
    STERANKA, LR
    [J]. MEDICINAL RESEARCH REVIEWS, 1990, 10 (02) : 237 - 269
  • [8] CHAI KX, 1991, J BIOL CHEM, V266, P16029
  • [9] CLONING OF THE GENE AND CDNA FOR MAMMALIAN BETA-ADRENERGIC-RECEPTOR AND HOMOLOGY WITH RHODOPSIN
    DIXON, RAF
    KOBILKA, BK
    STRADER, DJ
    BENOVIC, JL
    DOHLMAN, HG
    FRIELLE, T
    BOLANOWSKI, MA
    BENNETT, CD
    RANDS, E
    DIEHL, RE
    MUMFORD, RA
    SLATER, EE
    SIGAL, IS
    CARON, MG
    LEFKOWITZ, RJ
    STRADER, CD
    [J]. NATURE, 1986, 321 (6065) : 75 - 79
  • [10] EFFECT OF CHANGES IN SODIUM OR WATER-INTAKE ON GLOMERULAR B2-KININ-BINDING SITES
    EMOND, C
    BASCANDS, JL
    CABOSBOUTOT, G
    PECHER, C
    GIROLAMI, JP
    [J]. AMERICAN JOURNAL OF PHYSIOLOGY, 1989, 257 (03): : F353 - F358