Frequent nonreciprocal translocations in the amphidiploid genome of oilseed rape (Brassica napus)

被引：202

作者：

Sharpe, AG ^{[1
]}

Parkin, IAP ^{[1
]}

Keith, DJ ^{[1
]}

Lydiate, DJ ^{[1
]}

机构：

[1] JOHN INNES CTR, NORWICH NR4 7UH, NORFOLK, ENGLAND

来源：

GENOME | 1995年 / 38卷 / 06期

关键词：

restriction fragment length polymorphism; genetic linkage map; homoeologous recombination; microspore culture; doubled haploid;

D O I：

10.1139/g95-148

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A RFLP map of Brassica napus, consisting of 277 loci arranged in 19 linkage groups, was produced from genetic segregation in a combined population of 174 doubled-haploid microspore-derived lines. The integration of this map with a B. napus map derived from a resynthesized B. napus X oilseed rape cross allowed the 10 linkage groups of the B. napus A genome and the 9 linkage groups of the C genome to be identified. Collinear patterns of marker loci on different linkage groups suggested potential partial homoeologues. RFLP patterns consistent with aberrant chromosomes were observed in 9 of the 174 doubled-haploid lines. At least 4 of these lines carried nonreciprocal, homoeologous translocations. These translocations were probably the result of homoeologous recombination in the amphidiploid genome of oilseed rape, suggesting that domesticated B. napus is unable to control chromosome pairing completely. Evidence for genome homogenization in oilseed rape is presented and its implications on genetic mapping in amphidiploid species is discussed. The level of polymorphism in the A genome was higher than that in the C genome and this might be a general property of oilseed rape crosses.

引用

页码：1112 / 1121

页数：10

共 33 条

[1] MEIOTIC PAIRING IN HAPLOIDS AND AMPHIDIPLOIDS OF SPONTANEOUS VERSUS SYNTHETIC ORIGIN IN RAPE, BRASSICA-NAPUS L [J].