PURIFICATION AND CHARACTERIZATION OF THE ALPHA-TOCOPHEROL TRANSFER PROTEIN FROM RAT-LIVER

被引：171

作者：

SATO, Y ^{[1
]}

HAGIWARA, K ^{[1
]}

ARAI, H ^{[1
]}

INOUE, K ^{[1
]}

机构：

[1] UNIV TOKYO, FAC PHARMACEUT SCI, DEPT ANALYT CHEM, TOKYO 113, JAPAN

来源：

FEBS LETTERS | 1991年 / 288卷 / 1-2期

关键词：

ALPHA-TOCOPHEROL; VITAMIN-E; TRANSFER PROTEIN;

D O I：

10.1016/0014-5793(91)80999-J

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Alpha-Tocopheral transfer protein was purified from the 10 000 x g supernatant of rat liver. Two isoforms of the transfer protein exist, of which the isoelectric points are 5.0 and 5.1 as determined by chromatofocusing. These two isoforms have the same molecular weight; both showed molecular weight of approx. 30 500 on SDS-polyacrylamide gel electrophoresis. They cannot be distinguished from each other by amino acid composition or substrate specificity.

引用

页码：41 / 45

页数：5

共 10 条

[1]

BEHRENS WA, 1982, NUTR REP INT, V25, P107

[2]

BLOJ B, 1977, J BIOL CHEM, V252, P1613

[3] RAT-LIVER ALPHA-TOCOPHEROL BINDING-PROTEIN [J].

CATIGNANI, GL ;

BIERI, JG .

BIOCHIMICA ET BIOPHYSICA ACTA, 1977, 497 (02) :349-357

[4] ALPHA-TOCOPHEROL BINDING-PROTEIN IN RAT-LIVER CYTOPLASM [J].

CATIGNANI, GL .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1975, 67 (01) :66-72

[5]

KAPLOWITZ N, 1989, ANN NY ACAD SCI, V570, P85

[6]

LOWRY OH, 1951, J BIOL CHEM, V193, P265

[7]

MOWRI H, 1981, EUR J BIOCHEM, V117, P537

[8]

MURPHY DJ, 1981, J BIOL CHEM, V256, P464

[9]

OFARRELL PH, 1975, J BIOL CHEM, V250, P4007

[10] OCCURRENCE OF ALPHA-TOCOPHEROL BINDING PROTEIN IN RAT-LIVER CELL SAP [J].

RAJARAM, OV ;

FATTERPA.P ;

SREENIVA.A .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1973, 52 (02) :459-465

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