MASS-SPECTROMETRIC STUDIES ON THE N-LINKED OLIGOSACCHARIDES OF BACULOVIRUS-EXPRESSED MOUSE INTERLEUKIN-3

被引：16

作者：

HOGELAND, KE

DEINZER, ML

机构：

[1] OREGON STATE UNIV, DEPT CHEM, CORVALLIS, OR 97331 USA

[2] OREGON STATE UNIV, DEPT AGR CHEM, CORVALLIS, OR 97331 USA

来源：

BIOLOGICAL MASS SPECTROMETRY | 1994年 / 23卷 / 04期

关键词：

D O I：

10.1002/bms.1200230406

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

A combination of mass spectrometry techniques, high-pH anion-exchange chromatography and enzymatic digestions has been applied to elucidate the structures of the N-linked oligosaccharides on baculovirus-expressed mouse interleukin-3 produced in Bombyx mori larvae. This recombinant lymphokine was found to be heterogeneously glycosylated with mannosidic chitobiose core type oligosaccharides. A single N-linked oligosaccharide with the truncated structure of Man alpha 1-1-6Man beta 1-4GlcNAc1-4(Fuc alpha 1-6)GlcNAc was found to be the predominant species.

引用

收藏

页码：218 / 224

页数：7

相关论文

共 26 条

[1] ANALYSIS OF GLYCOPROTEIN-DERIVED OLIGOSACCHARIDES BY HIGH-PH ANION-EXCHANGE CHROMATOGRAPHY [J].

BASA, LJ ;

SPELLMAN, MW .

JOURNAL OF CHROMATOGRAPHY, 1990, 499 :205-220

[2] CENTRIFUGAL SIZE-EXCLUSION CHROMATOGRAPHIC METHOD FOR RAPID DESALTING AND FILTERING OF CARBOHYDRATE SAMPLES PRIOR TO FAST-ATOM-BOMBARDMENT MASS-SPECTROMETRY [J].

CHEN, TF ;

YU, HN ;

BAROFSKY, DF .

ANALYTICAL CHEMISTRY, 1992, 64 (17) :2014-2019

[3] A SIMPLE AND RAPID METHOD FOR THE PERMETHYLATION OF CARBOHYDRATES [J].

CIUCANU, I ;

KEREK, F .

CARBOHYDRATE RESEARCH, 1984, 131 (02) :209-217

[4]

CLARKLEWIS I, 1984, J BIOL CHEM, V259, P7488

[5] FAB-MASS SPECTROMETRY OF CARBOHYDRATES [J].

DELL, A .

ADVANCES IN CARBOHYDRATE CHEMISTRY AND BIOCHEMISTRY, 1987, 45 :19-72

[6] FAST-ATOM-BOMBARDMENT MASS-SPECTROMETRY FOR STRUCTURAL ELUCIDATION OF GLYCOCONJUGATES [J].

EGGE, H ;

PETERKATALINIC, J .

MASS SPECTROMETRY REVIEWS, 1987, 6 (03) :331-393

[7] MOLECULAR-CLONING OF CDNA FOR MURINE INTERLEUKIN-3 [J].

FUNG, MC ;

HAPEL, AJ ;

YMER, S ;

COHEN, DR ;

JOHNSON, RM ;

CAMPBELL, HD ;

YOUNG, IG .

NATURE, 1984, 307 (5948) :233-237

[8] MONOSACCHARIDE ANALYSIS OF GLYCOCONJUGATES BY ANION-EXCHANGE CHROMATOGRAPHY WITH PULSED AMPEROMETRIC DETECTION [J].

HARDY, MR ;

TOWNSEND, RR ;

LEE, YC .

ANALYTICAL BIOCHEMISTRY, 1988, 170 (01) :54-62

[9] SEPARATION OF POSITIONAL ISOMERS OF OLIGOSACCHARIDES AND GLYCOPEPTIDES BY HIGH-PERFORMANCE ANION-EXCHANGE CHROMATOGRAPHY WITH PULSED AMPEROMETRIC DETECTION [J].

HARDY, MR ;

TOWNSEND, RR .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (10) :3289-3293

[10] LIQUID SECONDARY ION MASS-SPECTROMETRY ANALYSIS OF PERMETHYLATED, N-HEXYLAMINE DERIVATIZED OLIGOSACCHARIDES - APPLICATION TO BACULOVIRUS EXPRESSED MOUSE INTERLEUKIN-3 [J].

HOGELAND, KE ;

ARBOGAST, B ;

DEINZER, ML .

JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 1992, 3 (04) :345-352