PURIFICATION OF SOYBEAN TRYPSIN-INHIBITORS BY MEANS OF CHROMATOGRAPHIC METHODS

The paper presents comparative studies on chromatographic purification of soybean trypsin inhibitors (STI) applying various polymer matrices. Two types of copolymers were tested: a copolymer of butyl acrylate with ethylene glycol dimethacrylate, and a terpolymer of acrylonitrile and butyl acrylate crosslinked with divinylbenzene. The investigations revealed that a similar purification extent is achieved in affinity chromatography with trypsin attached to both copolymers and in ion exchange chromatography when the matrices are aminated with ethylene diamine. The purification phenomenon was not so high for both unmodified matrices, and quite negligible for matrices hydrophilized by end-capping with tris(hydroxymethyl)aminomethane.