BIOSYNTHESIS OF SULPHOQUINOVOSYLDIACYLGLYCEROL BY CHLOROPLAST FRACTIONS FROM PEA AND LETTUCE

被引：9

作者：

PUGH, CE

HAWKES, T

HARWOOD, JL

机构：

[1] UNIV WALES COLL CARDIFF,SCH MOLEC & MED BIOSCI,CARDIFF CF1 3US,S GLAM,WALES

[2] ZENECA AGROCHEM,JEALOTTS HILL RES STN,BRACKNELL RG12 6EY,BERKS,ENGLAND

来源：

PHYTOCHEMISTRY | 1995年 / 39卷 / 05期

基金：

英国生物技术与生命科学研究理事会;

关键词：

PISUM SATIVUM; LEGUMINOSAE; LACTUCA SATIVA; COMPOSITAE; SULPHOLIPID; SULPHOQUINOVOSYLDIACYLGLYCEROL; BIOSYNTHESIS; ISOLATED CHLOROPLASTS;

D O I：

10.1016/0031-9422(95)00061-B

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Chloroplasts isolated from pea and lettuce leaves were found to synthesize sulphoquinovosyldiacylglycerol from [S-35]sulphate. Optimal conditions were defined for this synthesis. Rates of up to 1.3 and 0.8 nmol mg chlorophyll(-1) hr(-1) (at pH 7.9) were obtained for pea and lettuce chloroplasts, respectively. Synthesis by intact chloroplasts was stimulated by UTP (uridine triphosphate) and was light-dependent. The rates of synthesis achieved in vitro were sufficient to show that the chloroplast is autonomous with respect to synthesis of sulpholipid. The ready availability of pea leaves and the ease of isolation and activity of their chloroplasts make such preparations very suitable for detailed studies of sulpholipid synthesis.

引用

页码：1071 / 1075

页数：5

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