PHENOLIC ACRIDINE-ORANGE FLUORESCENT STAIN FOR MYCOBACTERIA

被引：21

作者：

SMITHWICK, RW

BIGBIE, MR

FERGUSON, RB

KARLIX, MA

WALLIS, CK

机构：

[1] TENNESSEE DEPT HLTH,LAB SERV,NASHVILLE,TN 37219

[2] NEW MEXICO DEPT HLTH,DIV SCI LAB,ALBUQUERQUE,NM 87106

[3] INDIANA STATE BOARD HLTH,DIV DIS CONTROL LAB,INDIANAPOLIS,IN 46206

[4] UNIV WASHINGTON,HARBORVIEW MED CTR,SEATTLE,WA 98104

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 1995年 / 33卷 / 10期

关键词：

D O I：

10.1128/JCM.33.10.2763-2764.1995

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A new fluorescence acid-fast staining method with acridine orange as the specific stain is presented. Only two reagents are required: the acridine orange-specific stain and a destaining-counterstaining reagent. Compared with auramine fluorescence acid-fast staining, there was less nonspecific staining of non-acid-fast debris which fluoresced a pale green contrasting color to provide a background in which to search for the red-to-orange fluorescing acid-fast bacilli. The results of the study indicate that the acridine orange method is superior to the auramine method in detecting acid-fast bacilli in specimen smears.

引用

页码：2763 / 2764

页数：2

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