URACIL-DNA GLYCOSYLASE ACTIVITY FROM DICTYOSTELIUM-DISCOIDEUM

被引：12

作者：

GUYER, RB ^{[1
]}

NONNEMAKER, JM ^{[1
]}

DEERING, RA ^{[1
]}

机构：

[1] PENN STATE UNIV, DEPT MOLEC & CELLULAR BIOL, 201 ALTHOUSE LAB, UNIVERSITY PK, PA 16802 USA

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1986年 / 868卷 / 04期

关键词：

D O I：

10.1016/0167-4781(86)90063-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have isolated and partially characterized a uracil-DNA glycosylase activity from the cellular slime mold, Dictyostelium discoideum. This glycosylase has a broad pH optimum (6.5-8.5) and is fully active in 10 mM EDTA or in 5 mM Mg2+. Its molecular weight by gel filtration is about 55000. This enzyme activity may work in concert with previously described apurinic/apyrimidinic (AP) endonuclease activities in the excision repair of uracil from the DNA of this lower eukaryote.

引用

页码：262 / 264

页数：3

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