A MONOCLONAL BLOCKING ELISA DETECTING SERUM ANTIBODIES TO MYCOPLASMA-HYOPNEUMONIAE

被引:74
作者
FELD, NC
QVIST, P
AHRENS, P
FRIIS, NF
MEYLING, A
机构
[1] National Veterinary Laboratory, DK-1503 Copenhagen V
关键词
D O I
10.1016/0378-1135(92)90092-8
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
A monoclonal blocking enzyme-linked immunosorbent assay (ELISA) for detection of antibodies to Mycoplasma hyopneumoniae in porcine serum has been developed. The monoclonal antibody (mAb) reacts with an M. hyopneumoniae specific epitope on a molecule of approximately 74 kDa. Only sera from M. hyopneumoniae infected pigs were able to block the binding of the mAb although antibodies from M. flocculare infected pigs also recognized a 74 kDa molecule. Sera from experimentally infected pigs as well as field samples were compared by the ELISA and by an indirect hemagglutination assay (IHA). In experimental pigs, the earliest detectable antibody response was found to be almost identical for both assays, but for some of the pigs the time of detection was significantly earlier by blocking ELISA than by IHA. In naturally infected herds more samples were found to be positive by ELISA than by IHA. Furthermore, the results indicate that sera from naturally M. flocculare infected pigs may give rise to cross-reactions in the IHA. The blocking ELISA appears to be a valuable and reproducible tool in the surveillance and serodiagnosis of M. hyopneumoniae infections in pigs.
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页码:35 / 46
页数:12
相关论文
共 28 条
[1]  
ARMSTRONG CH, 1987, CAN J VET RES, V51, P185
[2]  
ARMSTRONG CH, 1983, CAN J COMP MED, V47, P464
[3]  
ASTALDI GCB, 1980, J IMMUNOL, V125, P1411
[4]   THE 75-KILODALTON CYTOPLASMIC CHLAMYDIA-TRACHOMATIS L2 POLYPEPTIDE IS A DNAK-LIKE PROTEIN [J].
BIRKELUND, S ;
LUNDEMOSE, AG ;
CHRISTIANSEN, G .
INFECTION AND IMMUNITY, 1990, 58 (07) :2098-2104
[5]   NATIVE IMMUNOBLOTTING - TRANSFER OF MEMBRANE-PROTEINS IN THE PRESENCE OF NONIONIC DETERGENT [J].
BJERRUM, OJ ;
SELMER, JC ;
LIHME, A .
ELECTROPHORESIS, 1987, 8 (09) :388-397
[6]   SPECIES-SPECIFIC ANTIGENS OF MYCOPLASMA-HYOPNEUMONIAE AND CROSS-REACTIONS WITH OTHER PORCINE MYCOPLASMAS [J].
BOLSKE, G ;
STRANDBERG, ML ;
BERGSTROM, K ;
JOHANSSON, KE .
CURRENT MICROBIOLOGY, 1987, 15 (04) :233-239
[7]  
BOULANGE.P, 1968, CAN J COMPARAT MED, V32, P547
[8]   QUANTITATIVE DETECTION OF ANTIBODIES TO MYCOPLASMA-SUIPNEUMONIAE IN PIGS SERA BY AN ENZYME-LINKED IMMUNOSORBENT ASSAY [J].
BRUGGMANN, S ;
KELLER, H ;
BERTSCHINGER, HU ;
ENGBERG, B .
VETERINARY RECORD, 1977, 101 (06) :109-111
[9]  
FRIIS NF, 1977, ACTA VET SCAND, V18, P168
[10]  
FRIIS NF, 1984, ACTA VET SCAND, V25, P29