NUCLEOTIDE-SEQUENCE AND CHARACTERIZATION OF ERYTHROMYCIN RESISTANCE DETERMINANT THAT ENCODES MACROLIDE 2'-PHOSPHOTRANSFERASE-I IN ESCHERICHIA-COLI

被引：82

作者：

NOGUCHI, N

EMURA, A

MATSUYAMA, H

OHARA, K

SASATSU, M

KONO, M

机构：

[1] Department of Microbiology, School of Pharmacy, Tokyo Univ. of Pharmacy/Life Science, Hachioji, Tokyo 192-03

来源：

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 1995年 / 39卷 / 10期

关键词：

D O I：

10.1128/AAC.39.10.2359

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The DNA fragment (3.3 kb) containing the erythromycin resistance determinant was cloned from Escherichia coli Tf481A and sequenced. Deletion and complementation analyses indicated that the expression of high-level resistance to erythromycin requires two genes, mphA and mrx, which encode macrolide 2'-phosphotransferase I and an unidentified hydrophobic protein, respectively.

引用

页码：2359 / 2363

页数：5

共 19 条

[1] CONSTRUCTION AND CHARACTERIZATION OF AMPLIFIABLE MULTICOPY DNA CLONING VEHICLES DERIVED FROM P15A CRYPTIC MINIPLASMID [J].

CHANG, ACY ;

COHEN, SN .

JOURNAL OF BACTERIOLOGY, 1978, 134 (03) :1141-1156

[2] TRANSLATIONAL INITIATION IN PROKARYOTES [J].

GOLD, L ;

PRIBNOW, D ;

SCHNEIDER, T ;

SHINEDLING, S ;

SINGER, BS ;

STORMO, G .

ANNUAL REVIEW OF MICROBIOLOGY, 1981, 35 :365-403

[3] PROPERTIES OF AN R FACTOR WHICH ORIGINATED IN PSEUDOMONAS-AERUGINOSA 1822 [J].

GRINSTED, J ;

SAUNDERS, JR ;

SYKES, RB ;

RICHMOND, MH ;

INGRAM, LC .

JOURNAL OF BACTERIOLOGY, 1972, 110 (02) :529-&

[4] RESTRICTION ENZYME MAP OF R-PLASMID RP1 [J].

GRINSTED, J ;

BENNETT, PM ;

RICHMOND, MH .

PLASMID, 1977, 1 (01) :34-37

[5]

HENIKOFF S, 1987, GENE, V28, P351

[6] THE KANAMYCIN RESISTANCE TRANSPOSON TN2680 DERIVED FROM THE R-PLASMID RTS1 AND CARRIED BY PHAGE-P1KM HAS FLANKING 0.8-KB-LONG DIRECT REPEATS [J].

IIDA, S ;

MEYER, J ;

LINDER, P ;

GOTO, N ;

NAKAYA, R ;

REIF, HJ ;

ARBER, W .

PLASMID, 1982, 8 (02) :187-198

[7]

KUNKEL TA, 1987, METHOD ENZYMOL, V154, P367

[8] A SIMPLE METHOD FOR DISPLAYING THE HYDROPATHIC CHARACTER OF A PROTEIN [J].

KYTE, J ;

DOOLITTLE, RF .

JOURNAL OF MOLECULAR BIOLOGY, 1982, 157 (01) :105-132

[9] TN1525, A KANAMYCIN-R DETERMINANT FLANKED BY 2 DIRECT COPIES OF IS15 [J].

LABIGNEROUSSEL, A ;

BRIAUXGERBAUD, S ;

COURVALIN, P .

MOLECULAR AND GENERAL GENETICS, 1983, 189 (01) :90-101

[10]

MORMORE WN, 1976, GUANINE PLUS CYTOSIN

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