PHASE-SEPARATION WITHIN ARTIFICIAL GRANULES FROM A BLEND OF POLYHYDROXYBUTYRATE AND POLYHYDROXYOCTANOATE - BIOLOGICAL IMPLICATIONS

被引：13

作者：

HOROWITZ, DM ^{[1
]}

SANDERS, JKM ^{[1
]}

机构：

[1] UNIV CAMBRIDGE,CHEM LAB,CAMBRIDGE CB2 1EW,ENGLAND

来源：

POLYMER | 1994年 / 35卷 / 23期

关键词：

POLY(3-HYDROXYBUTYRATE); POLY(3-HYDROXYOCTANOATE); ARTIFICIAL GRANULES);

D O I：

10.1016/0032-3861(94)90668-8

中图分类号：

O63 [高分子化学（高聚物）];

学科分类号：

070305 ; 080501 ; 081704 ;

摘要：

A recently developed in vitro o procedure for preparing artificial granules of microbial polyesters has been applied to a blend of poly(3-hydroxybutyrate) (PHB) and poly(3-hydroxyoctanoate) (PHO). Density gradient analysis showed that these granules formed a single, uniform population; n.m.r. showed that the polymers retained their amorphous elastomeric state; and thermal analysis indicated that the polymers formed separate phases within these granules. These results contrast strongly with the observation that a recombinant bacterial strain simultaneously expressing PHB and PHO stores the two polymers in separate cellular granules. The implications of the in vitro findings for granule formation in vivo are discussed.

引用

页码：5079 / 5083

页数：5

共 25 条

[1] OCCURRENCE, METABOLISM, METABOLIC ROLE, AND INDUSTRIAL USES OF BACTERIAL POLYHYDROXYALKANOATES [J].