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INDIRECT FLUOROMETRIC DETECTION OF CATIONS IN CAPILLARY ZONE ELECTROPHORESIS

被引：124
作者：
GROSS, L
YEUNG, ES
机构：
[1] IOWA STATE UNIV SCI & TECHNOL,US DOE,AMES LAB,AMES,IA 50011
[2] IOWA STATE UNIV SCI & TECHNOL,DEPT CHEM,AMES,IA 50011
来源：
ANALYTICAL CHEMISTRY | 1990年 / 62卷 / 05期
关键词：
D O I：
10.1021/ac00204a002
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A new method for detecting cations separated by capillary zone electrophoresis is described. Quinine sulfate is a useful buffer for indirect fluorescence detection. The positively charged, fluorescent quinine ion is displaced by nonfluores-cent solute cations. In contrast to previous work with anions, quinine Ion adsorbs to the capillary wall. The effect of adsorption on resolution and reproducibility is discussed. Applications are presented for mono- and divalent cations, amines, and oligopeptides. Because small Internal diameter capillaries can be used for this technique, very low quantities of cations injected can be detected. © 1990, American Chemical Society. All rights reserved.
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页码：427 / 431
页数：5
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