THE PHORBOL ESTER TPA INDUCES A TRANSLOCATION OF THE INSULIN SENSITIVE GLUCOSE CARRIER (GLUT4) IN FAT-CELLS

被引：16

作者：

VOGT, B ^{[1
]}

MUSHACK, J ^{[1
]}

SEFFER, E ^{[1
]}

HARING, HU ^{[1
]}

机构：

[1] INST DIABET FORSCH MUNCHEN,KOLNER PL 1,W-8000 MUNICH 40,GERMANY

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1990年 / 168卷 / 03期

关键词：

D O I：

10.1016/0006-291X(90)91141-E

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Insulin activates the glucose transport in isolated fat cells through a translocation of the insulin sensitive glucose carrier subtype (GLUT4) and by activation of glucose carriers in the plasma membrane. Protein kinase C stimulating phorbol esters are able to mimick partially the insulin effect on glucose transport. In order to determine whether this phorbol ester effect occurs through a translocation of the insulin sensitive glucose carrier (GLUT4) we used a monoclonal antibody against GLUT4 to determine its distribution in subcellular fractions of rat adipocytes. We found that the phorbol ester TPA is able to increase the amount of GLUT4 in the plasma membrane fraction about two-fold. © 1990.

引用

页码：1089 / 1094

页数：6

共 17 条

[1] POLYMORPHIC HUMAN INSULIN-RESPONSIVE GLUCOSE-TRANSPORTER GENE ON CHROMOSOME 17P13 [J].

BELL, GI ;

MURRAY, JC ;

NAKAMURA, Y ;

KAYANO, T ;

EDDY, RL ;

FAN, YS ;

BYERS, MG ;

SHOWS, TB .

DIABETES, 1989, 38 (08) :1072-1075

[2] CLONING AND CHARACTERIZATION OF A CDNA-ENCODING THE RAT-BRAIN GLUCOSE-TRANSPORTER PROTEIN [J].

BIRNBAUM, MJ ;

HASPEL, HC ;

ROSEN, OM .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (16) :5784-5788

[3]

FUKUMOTO H, 1989, J BIOL CHEM, V264, P7776

[4] COUPLING OF INSULIN BINDING AND INSULIN ACTION ON GLUCOSE-TRANSPORT IN FAT-CELLS [J].

HARING, HU ;

BIERMANN, E ;

KEMMLER, W .

AMERICAN JOURNAL OF PHYSIOLOGY, 1981, 240 (05) :E556-E565

[5] INSULIN-REGULATABLE TISSUES EXPRESS A UNIQUE INSULIN-SENSITIVE GLUCOSE-TRANSPORT PROTEIN [J].

JAMES, DE ;

BROWN, R ;

NAVARRO, J ;

PILCH, PF .

NATURE, 1988, 333 (6169) :183-185

[6] MOLECULAR-CLONING AND CHARACTERIZATION OF AN INSULIN-REGULATABLE GLUCOSE TRANSPORTER [J].

JAMES, DE ;

STRUBE, M ;

MUECKLER, M .

NATURE, 1989, 338 (6210) :83-87

[7]

KARNIELI E, 1981, J BIOL CHEM, V256, P4772

[8] PHORBOLESTERS ENHANCE BASAL D-GLUCOSE TRANSPORT BUT INHIBIT INSULIN STIMULATION OF D-GLUCOSE TRANSPORT AND INSULIN BINDING IN ISOLATED RAT ADIPOCYTES [J].

KIRSCH, D ;

OBERMAIER, B ;

HARING, HU .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1985, 128 (02) :824-832

[9] CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4 [J].

LAEMMLI, UK .

NATURE, 1970, 227 (5259) :680-+

[10] PREPARATION AND CHARACTERIZATION OF A PLASMA MEMBRANE FRACTION FROM ISOLATED FAT CELLS [J].

MCKEEL, DW ;

JARETT, L .

JOURNAL OF CELL BIOLOGY, 1970, 44 (02) :417-&

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