EXAMINATION OF NEW STATIONARY PHASES FOR THE REVERSED-PHASE CHROMATOGRAPHY OF PEPTIDES

被引：10

作者：

REH, E

KAPFER, U

机构：

[1] Boehringer Mannheim GmbH, Tutzing, D-8132

来源：

CHROMATOGRAPHIA | 1990年 / 30卷 / 11-12期

关键词：

COLUMN LIQUID CHROMATOGRAPHY; NEW STATIONARY PHASES; PEPTIDES; PERFORMANCE TESTING;

D O I：

10.1007/BF02269742

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Some new HPLC-phases (Polyencap, Hypercarb, Poly F, PRP-infinity) have been assessed for their suitability in the separation of peptides. Test criteria were peptide recovery, separation-efficiency, selectivity and stability. The eluents TFA/acetonitrile and acetate-buffer/propanol are compared. Of interest are the stability and recovery of Polyencap, the high separation efficiency of the standard RP-8 silica-based phase (Nucleosil C8) and the unusual peak-width of PRP-infinity. Acceptable conditions for peptide separations may be provided by Polyencap, but stability still has to be improved.

引用

页码：663 / 674

页数：12

共 12 条

[1] COMPARISON OF REVERSED-PHASE COLUMNS FOR THE SEPARATION OF TRYPTIC PEPTIDES BY GRADIENT ELUTION - CORRELATION OF EXPERIMENTAL RESULTS AND MODEL PREDICTION [J].