REGULATION OF WATER-FLOW BY ACTIN-BINDING PROTEIN-INDUCED ACTIN GELATION

被引：30

作者：

ITO, T

SUZUKI, A

STOSSEL, TP

机构：

[1] HARVARD UNIV,BRIGHAM & WOMENS HOSP,SCH MED,DEPT MED,DIV EXPTL MED,BOSTON,MA 02115

[2] HARVARD UNIV,BRIGHAM & WOMENS HOSP,SCH MED,DEPT MED,DIV HEMATOL ONCOL,BOSTON,MA 02115

来源：

BIOPHYSICAL JOURNAL | 1992年 / 61卷 / 05期

关键词：

D O I：

10.1016/S0006-3495(92)81938-0

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

Actin filaments inhibit osmotically driven water flow (Ito, T., K.S. Zaner, and T.P. Stossel. 1987. Biophys. J.51:745-753). Here we show that the actin gelation protein, actin-binding protein (ABP), impedes both osmotic shrinkage and swelling of an actin filament solution and reduces markedly the concentration of actin filaments required for this inhibition. These effects depend on actin filament immobilization, because the ABP concentration that causes initial impairment of water flow by actin filaments corresponds to the gel point measured viscometrically and because gelsolin, which noncovalently severs actin filaments, solates actin gels and restores water flow in a solution of actin cross-linked by ABP. Since ABP gels actin filaments in the periphery of many eukaryotic cells, such actin networks may contribute to physiological cell volume regulation.

引用

页码：1301 / 1305

页数：5

共 17 条

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