EVIDENCE THAT INSULIN ACTIVATES CASEIN KINASE-2 IN RAT EPIDIDYMAL FAT-CELLS AND THAT THIS MAY RESULT IN THE INCREASED PHOSPHORYLATION OF AN ACID-SOLUBLE 22-KDA PROTEIN

被引：26

作者：

DIGGLE, TA ^{[1
]}

SCHMITZPEIFFER, C ^{[1
]}

BORTHWICK, AC ^{[1
]}

WELSH, GI ^{[1
]}

DENTON, RM ^{[1
]}

机构：

[1] UNIV BRISTOL,SCH MED,DEPT BIOCHEM,UNIV WALK,BRISTOL BS8 1TD,AVON,ENGLAND

来源：

BIOCHEMICAL JOURNAL | 1991年 / 279卷

关键词：

D O I：

10.1042/bj2790545

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Casein kinase 2 activity as measured by phosphorylation of the peptide substrate Arg-Arg-Arg-Glu-Glu-Glu-Thr-Glu-Glu-Glu is increased by about 50% in extracts from insulin-treated epididymal fat-pads or isolated fat-cells after purification by Mono Q chromatography. Insulin acts to increase the V(max.) of the kinase. An acid-soluble protein with an apparent subunit molecular mass of about 22 kDa appears to be a substrate for casein kinase 2. The protein possesses a number of properties in common with the acid-soluble heat-stable 22 kDa protein which exhibits increased phosphorylation in rat adipose tissue exposed to insulin.

引用

页码：545 / 551

页数：7

共 44 条

[1] STIMULATION OF CASEIN KINASE-II BY EPIDERMAL GROWTH-FACTOR - RELATIONSHIP BETWEEN THE PHYSIOLOGICAL-ACTIVITY OF THE KINASE AND THE PHOSPHORYLATION STATE OF ITS BETA-SUBUNIT [J].

ACKERMAN, P ;

GLOVER, CVC ;

OSHEROFF, N .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (02) :821-825

[2] REGULATION OF CASEIN KINASE-2 BY PHOSPHORYLATION DEPHOSPHORYLATION [J].

AGOSTINIS, P ;

GORIS, J ;

PINNA, LA ;

MERLEVEDE, W .

BIOCHEMICAL JOURNAL, 1987, 248 (03) :785-789

[3]

AHMAD Z, 1982, J BIOL CHEM, V257, P5873

[4]

AHN NG, 1990, J BIOL CHEM, V265, P11487

[5]

ALEXANDER MC, 1982, J BIOL CHEM, V257, P2049

[6]

BELSHAM G J, 1980, Biochemical Society Transactions, V8, P382

[7] REVERSIBILITY OF THE INSULIN-STIMULATED PHOSPHORYLATION OF ATP CITRATE LYASE AND A CYTOPLASMIC PROTEIN OF SUBUNIT MR 22000 IN ADIPOSE-TISSUE [J].

BELSHAM, GJ ;

BROWNSEY, RW ;

DENTON, RM .

BIOCHEMICAL JOURNAL, 1982, 204 (01) :345-352

[8] PRELIMINARY CHARACTERIZATION OF A HEAT-STABLE PROTEIN FROM RAT ADIPOSE-TISSUE WHOSE PHOSPHORYLATION IS STIMULATED BY INSULIN [J].

BLACKSHEAR, PJ ;

NEMENOFF, RA ;

AVRUCH, J .

BIOCHEMICAL JOURNAL, 1982, 204 (03) :817-824

[9] PROTEIN-SERINE KINASE FROM RAT EPIDIDYMAL ADIPOSE-TISSUE WHICH PHOSPHORYLATES AND ACTIVATES ACETYL-COA CARBOXYLASE - POSSIBLE ROLE IN INSULIN ACTION [J].

BORTHWICK, AC ;

EDGELL, NJ ;

DENTON, RM .

BIOCHEMICAL JOURNAL, 1990, 270 (03) :795-801

[10]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

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