LOCALIZATION OF A 210-KDA MICROTUBULE-INTERACTING PROTEIN IN THE YEAST SACCHAROMYCES-CEREVISIAE

被引:10
作者
HASEK, J
JOCHOVA, J
DRABER, P
VIKLICKY, V
STREIBLOVA, E
机构
[1] CZECHOSLOVAK ACAD SCI, INST MOLEC GENET, CS-11142 PRAGUE 1, CZECHOSLOVAKIA
[2] CZECHOSLOVAK ACAD SCI, INST MICROBIOL, CS-11142 PRAGUE 1, CZECHOSLOVAKIA
关键词
IMMUNOBLOTTING; IMMUNOFLUORESCENCE; MICROTUBULE-INTERACTING PROTEIN; SACCHAROMYCES-CEREVISIAE;
D O I
10.1139/m92-024
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Using the monoclonal antibody MA-01, which recognizes a 210-kDa protein in cell-free extracts, spindle and cytoplasmic microtubules were visualized in budding yeast, Saccharomyces cerevisiae. In additional, a spot-like staining was found beneath the plasma membrane, revealing in part correlation with F-actin distribution. This pattern was common for cells of all cell-cycle stages. The interaction of the protein recognized by MA-01 with microtubules was confirmed in the double labeling with a polyclonal antitubulin antibody and by the sensitivity of intranuclear structures stained by MA-01 to the microtubule disrupting drug nocodazole.
引用
收藏
页码:149 / 152
页数:4
相关论文
共 26 条
[1]  
AIZAWA H, 1989, J BIOL CHEM, V264, P5885
[2]   The cytoskeleton of Saccharomyces cerevisiae [J].
Barnes, G. ;
Drubin, D. G. ;
Stearns, T. .
CURRENT OPINION IN CELL BIOLOGY, 1990, 2 (01) :109-115
[3]   A MARGINAL BAND-ASSOCIATED PROTEIN HAS PROPERTIES OF BOTH MICROTUBULE-ASSOCIATED AND MICROFILAMENT-ASSOCIATED PROTEINS [J].
BIRGBAUER, E ;
SOLOMON, F .
JOURNAL OF CELL BIOLOGY, 1989, 109 (04) :1609-1620
[4]  
Byers B, 1981, MOL BIOL YEAST SACCH, P59
[5]  
CHAPIN SJ, 1991, J CELL SCI, V98, P27
[6]   HETEROGENEITY OF TUBULIN EPITOPES IN MOUSE FETAL TISSUES [J].
DRABER, P ;
LAGUNOWICH, LA ;
DRABEROVA, E ;
VIKLICKY, V ;
DAMJANOV, I .
HISTOCHEMISTRY, 1988, 89 (05) :485-492
[7]   CELLULAR-DISTRIBUTION OF A PROTEIN RELATED TO NEURONAL MICROTUBULE-ASSOCIATED PROTEIN MAP-2 IN LEYDIG-CELLS [J].
DRABEROVA, E ;
DRABER, P ;
VIKLICKY, V .
CELL BIOLOGY INTERNATIONAL REPORTS, 1986, 10 (11) :881-890
[8]  
HASEK J, 1987, J GEN MICROBIOL, V133, P3355
[9]  
HASEK J, 1986, EUR J CELL BIOL, V41, P150
[10]  
HAYLES J, 1986, J CELL SCI, P155