TYPE-II PHOSPHOLIPASE-A(2) IN HUMAN GESTATIONAL TISSUES - EXTRACTABLE IMMUNO- AND ENZYMATIC-ACTIVITY IN FETAL MEMBRANES

被引:32
作者
AITKEN, MA
FARRUGIA, W
WONG, MH
SCOTT, KF
BRENNECKE, SP
RICE, GE
机构
[1] MONASH UNIV,DEPT PHYSIOL,CLAYTON,VIC 3168,AUSTRALIA
[2] ST VINCENTS HOSP,DEPT MED,DARLINGHURST,NSW 2010,AUSTRALIA
基金
英国医学研究理事会;
关键词
PHOSPHOLIPASE-A2; CHORION; AMNION; PLACENTA; PARTURITION;
D O I
10.1016/0005-2760(93)90016-3
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
In this study, we have established the presence of immunoreactive (ir) Type II PLA2 in human amnion and choriodecidua obtained from women at term prior to the onset of labour. The content of irType II PLA2 present in 1 M NaCl extracts of choriodecidua and amnion averaged 3.5 +/- 3.1 and 10.6 +/- 5.2 ng/mg tissue protein (n = 3), respectively. PLA 2 enzymatic activity present in the same tissues averaged 1.3 +/- 0.2 and 1.9 +/- 0.7 nmol phosphatidylethanolamine (PE) hydrolysed/ mg tissue protein per h (n = 3), respectively. To allow intra-patient comparison of the relative distribution in gestational tissues, irType II PLA2 and PLA 2 enzymatic activity was also determined in placenta obtained from the same group of women, and averaged 26.0 +/- 7.0 ng/mg tissue protein and 3.5 +/- 1.0 nmol PE hydrolysed/mg protein per h (n = 3), respectively. As has been previously reported for human placenta, the recovery of Type II PLA2 and PLA2 enzymatic activity from amnion and choriodecidua was increased between 16- and 25-fold when tissues were homogenized in high-ionic strength media (i.e., 10% (w/v) ammonium sulphate or 1 M NaCl) compared with that recovered when tissues were homogenized in low-ionic strength media (i.e., 0.32 M sucrose-20 mM Hepes). The data obtained represent the first quantitative estimates of immunoreactive Type II PLA2 in human amnion and choriodecidua, and support the conclusion that previous analyses of the PLA2 enzymatic activity present in gestational tissues have essentially excluded the contribution made by this PLA2 isozyme to net enzymatic activity. We suggest that this isozyme represents a major component of the PLA2 enzymatic activity present in human gestational tissues at term and that it contributes significantly to the phospholipid metabolism and arachidonic acid release which occurs during late pregnancy and at the time of labour.
引用
收藏
页码:314 / 320
页数:7
相关论文
共 25 条
[1]   GESTATIONAL TISSUE PHOSPHOLIPASE-A2 MESSENGER-RNA CONTENT AND THE ONSET OF SPONTANEOUS LABOR IN THE HUMAN [J].
AITKEN, MA ;
RICE, GE ;
BRENNECKE, SP .
REPRODUCTION FERTILITY AND DEVELOPMENT, 1990, 2 (05) :575-580
[2]   RELATIVE ABUNDANCE OF HUMAN PLACENTAL PHOSPHOLIPASE-A2 MESSENGER-RNA IN LATE PREGNANCY [J].
AITKEN, MA ;
RICE, G ;
BRENNECKE, S .
PROSTAGLANDINS & OTHER LIPID MEDIATORS, 1992, 43 (04) :361-370
[3]   HUMAN PLACENTAL PHOSPHOLIPASE-A2 ACTIVITY IN TERM AND PRETERM LABOR [J].
BERNAL, AL ;
NEWMAN, GE ;
PHIZACKERLEY, PJR ;
BRYANTGREENWOOD, G ;
KEELING, JW .
EUROPEAN JOURNAL OF OBSTETRICS & GYNECOLOGY AND REPRODUCTIVE BIOLOGY, 1992, 43 (03) :185-192
[4]  
BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[5]   LINKING PHOSPHOLIPASE-A2 TO PHOSPHOLIPID TURNOVER AND PROSTAGLANDIN SYNTHESIS IN MAST-CELL GRANULES [J].
CHOCK, SP ;
RHEE, SG ;
TANG, LC ;
SCHMAUDERCHOCK, EA .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1991, 195 (03) :707-713
[6]   PURIFICATION OF A 110-KILODALTON CYTOSOLIC PHOSPHOLIPASE-A2 FROM THE HUMAN MONOCYTIC CELL-LINE U937 [J].
CLARK, JD ;
MILONA, N ;
KNOPF, JL .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (19) :7708-7712
[7]   EVOLUTIONARY RELATIONSHIPS AND IMPLICATIONS FOR THE REGULATION OF PHOSPHOLIPASE-A2 FROM SNAKE-VENOM TO HUMAN SECRETED FORMS [J].
DAVIDSON, FF ;
DENNIS, EA .
JOURNAL OF MOLECULAR EVOLUTION, 1990, 31 (03) :228-238
[8]  
DEHAAS GH, 1977, J BIOL CHEM, V252, P4913
[9]   TYPE-II PHOSPHOLIPASE-A(2) IN HUMAN GESTATIONAL TISSUES - SUBCELLULAR-DISTRIBUTION OF PLACENTAL IMMUNOACTIVITY AND CATALYTIC ACTIVITY [J].
FARRUGIA, W ;
AITKEN, MA ;
VANDUNNE, F ;
WONG, MH ;
BRENNECKE, SP ;
SCOTT, KF ;
RICE, GE .
BIOCHIMICA ET BIOPHYSICA ACTA, 1993, 1166 (01) :77-83
[10]  
FLOWER RJ, 1981, PROSTAGLANDIN SYSTEM, V36, P27