PURIFICATION OF RECOMBINANT RIBONUCLEASE-T1 EXPRESSED IN ESCHERICHIA-COLI

被引：39

作者：

SHIRLEY, BA

LAURENTS, DV

机构：

[1] Department of Biochemistry and Biophysics, Texas A and M University, College Station

来源：

JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS | 1990年 / 20卷 / 03期

关键词：

Absorption coefficient; purification; Ribonuclease T[!sub]1[!/sub;

D O I：

10.1016/0165-022X(90)90076-O

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A protocol for the rapid purification of ribonuclease T1 expressed from a chemically synthesized gene cloned into Escherichia coli is described. QAE ion-exchange and Sephadex G-50 chromatography are used to give over 300 mg (88% yield) of pure ribonuclease T1 from 6 1 of liquid culture in 3 days. We also report a new absorption coefficient for RNase T1: E278nm1% = 15.4. © 1990.

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页码：181 / 188

页数：8

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