MODULATION OF DIHYDROPYRIDINE-SENSITIVE CALCIUM CHANNELS - A ROLE FOR G-PROTEINS

被引：12

作者：

BERGAMASCHI, S

TRABUCCHI, M

BATTAINI, F

PARENTI, M

SCHETTINI, G

MEUCCI, O

GOVONI, S

机构：

[1] UNIV MILAN, INST PHARMACOL SCI, VIA BALZARETTI 9, I-20133 MILAN, ITALY

[2] UNIV ROME, CHAIR TOXICOL, I-00100 ROME, ITALY

[3] UNIV MILAN, DEPT PHARMACOL, I-20122 MILAN, ITALY

[4] NAPLES UNIV, DEPT PHARMACOL, I-80138 NAPLES, ITALY

[5] UNIV BARI, DEPT PHARMACOL, I-70124 BARI, ITALY

来源：

EUROPEAN NEUROLOGY | 1990年 / 30卷

关键词：

Dihydropyridines; G proteins; PC12; cells; Pertussis toxin; Voltage-dependent calcium channel;

D O I：

10.1159/000117186

中图分类号：

R74 [神经病学与精神病学];

学科分类号：

摘要：

The present study investigates the effect of G protein activation on dihydropyridine recognition sites in PC12 cell membranes. The addition of a stable analogue of GTP, GMP-PNP, increases the displacement of tritiated PN 200-110 produced by Bay K 8644 without modifying the one produced by nitrendipine. This effect is prevented by Pertussis toxin treatment. Functional studies based on the measurement of intracellular calcium concentrations by means of the fura 2 technique show that Pertussis toxin reduces the ability of Bay K.8644 to potentiate the increase of cytosolic calcium elicited by 80 mM K+. The results support the hypothesis that a G protein may modulate the activity of voltage-dependent, dihydropyridine-sensitive calcium channels. © 1990 S. Karger AG, Basel.

引用

页码：16 / 20

页数：5

共 21 条

[1] BINDING OF OMEGA-CONOTOXIN TO RECEPTOR-SITES ASSOCIATED WITH THE VOLTAGE-SENSITIVE CALCIUM-CHANNEL [J].