HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC METHOD FOR THE DETERMINATION OF SPECTINOMYCIN IN TURKEY PLASMA

被引：20

作者：

BURTON, SD ^{[1
]}

HUTCHINS, JE ^{[1
]}

FREDERICKSEN, TL ^{[1
]}

RICKS, C ^{[1
]}

TYCZKOWSKI, JK ^{[1
]}

机构：

[1] EMBREX INC,1035 SWABIA COURT,POB 13989,RES TRIANGLE PK,NC 27709

来源：

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1991年 / 571卷 / 1-2期

关键词：

D O I：

10.1016/0378-4347(91)80447-K

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A selective high-performance liquid chromatographic (HPLC) method with ultraviolet-visible (UV-VIS) detection was developed to measure therapeutic concentrations of spectinomycin in turkey plasma. Treatment of plasma samples with 3% trifluoroacetic acid in acetonitrile facilitated spectinomycin extraction and protein precipitation. After centrifugation, the stable derivatization reagent, 2,4-dinitrophenylhydrazine, was added to an aliquot of the supernatant, and the mixture was incubated for 30 min at 70-degrees-C. Excess reagent was quenched with acetone and additional heating. The resulting derivative, a proposed spectinomycin-hydrazone, was separated from other compounds by reversed-phase HPLC during a short gradient run. The absorbance of the effluent was monitored spectrophotometrically with the UV-VIS detector set at 205 nm. The detector response was linear through the range of interest, 2-100-mu-g/ml.

引用

页码：209 / 216

页数：8

共 7 条

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