REPRODUCING THE 3-DIMENSIONAL STRUCTURE OF A TRANSFER-RNA MOLECULE FROM STRUCTURAL CONSTRAINTS

被引：48

作者：

MAJOR, F ^{[1
]}

GAUTHERET, D ^{[1
]}

CEDERGREN, R ^{[1
]}

机构：

[1] UNIV MONTREAL, DEPT BIOCHIM, MONTREAL H3C 3J7, QUEBEC, CANADA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1993年 / 90卷 / 20期

关键词：

D O I：

10.1073/pnas.90.20.9408

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The three-dimensional structure of yeast tRNA(Phe) was reproduced at atomic resolution with the automated RNA modeling program MC-SYM, which is based on a constraint-satisfaction algorithm. Structural constraints used in the modeling were derived from the secondary structure, four tertiary base pairs, and other information available prior to the determination of the x-ray crystal structure of the tRNA. The program generated 26 solutions (models), all of which had the familiar ''L'' form of tRNA and root-mean-square deviations from the crystal structure in the range of 3.1-3.8 angstrom. The interaction between uridine-8 and adenosine-14 was crucial in the modelling procedure, since only this among the tertiary pairs is necessary and sufficient to reproduce the L form of tRNA. Other tertiary interactions were critical in reducing the number of solutions proposed by the program.

引用

页码：9408 / 9412

页数：5

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