NDF/HEREGULIN STIMULATES THE PHOSPHORYLATION OF HER3/ERBB3

被引：72

作者：

KITA, YA

BARFF, J

LUO, Y

WEN, DZ

BRANKOW, D

HU, S

LIU, NL

PRIGENT, SA

GULLICK, WJ

NICOLSON, M

机构：

[1] AMGEN INC, AMGEN CTR, DEPT MAMALIAN CELL MOLEC BIOL, THOUSAND OAKS, CA 91320 USA

[2] AMGEN INC, AMGEN CTR, DEPT PROC DEV, THOUSAND OAKS, CA 91320 USA

[3] UNIV CALIF SAN DIEGO, CTR CANC, SCH MED, DEPT MED, LA JOLLA, CA 92093 USA

[4] UNIV CALIF SAN DIEGO, CTR CANC, SCH MED, DEPT PHARMACOL, LA JOLLA, CA 92093 USA

[5] HAMMERSMITH HOSP, IMPERIAL CANC RES FUND, INCOL UNIT, LONDON W12 0NN, ENGLAND

来源：

FEBS LETTERS | 1994年 / 349卷 / 01期

关键词：

HER3; ERBB3; NDF; HEREGULIN; TYROSINE KINASE; EGF RECEPTOR FAMILY;

D O I：

10.1016/0014-5793(94)00644-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Her3/erbB3 has been identified as a third member of the epidermal growth factor receptor (EGFR) family [(1989) Proc. Natl. Acad. Sci. USA 86, 9193-9197; (1990) Proc. Natl. Acad. Sci. USA 87, 4905-4909]. The natural ligand for Her3 has not been identified. Although recently NDF has been proposed as a specific ligand for Her4 [(1993) Nature 366, 473-475; (1993) J. Biol. Chem. 268, 18407-18410], we report here that Her3 was phosphorylated on tyrosine not only in three breast carcinoma cell lines, MDAMB453, MDAMB468 and SKBR3, but also in Her3-transfected CHO cells in response to NDF stimulation. In further studies, cells were reacted with I-125-labeled NDF and then chemically crosslinked. Immunoprecipitation with anti-Her3 revealed a dense high M(W) band, greater than 400 kDa. The results suggest that NDF may be a ligand of Her3 and induces receptor hetero-oligomerization.

引用

页码：139 / 143

页数：5

共 32 条

[1] GROWTH-FACTORS AND CANCER
AARONSON, SA
[J]. SCIENCE, 1991, 254 (5035) : 1146 - 1153
[2] TUMOR PROMOTER AND EPIDERMAL GROWTH-FACTOR STIMULATE PHOSPHORYLATION OF THE C-ERBB-2-GENE PRODUCT IN MKN-7 HUMAN ADENOCARCINOMA CELLS
AKIYAMA, T
SAITO, T
OGAWARA, H
TOYOSHIMA, K
YAMAMOTO, T
[J]. MOLECULAR AND CELLULAR BIOLOGY, 1988, 8 (03) : 1019 - 1026
[3] CARPENTER G, 1985, J CELL SCI, P1
[4] CULOUSCOU JM, 1993, J BIOL CHEM, V268, P18407
[5] CHARACTERIZATION OF A NEU/C-ERBB-2 PROTEIN-SPECIFIC ACTIVATING FACTOR
DOBASHI, K
DAVIS, JG
MIKAMI, Y
FREEMAN, JK
HAMURO, J
GREENE, MI
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (19) : 8582 - 8586
[6] ARIA, A PROTEIN THAT STIMULATES ACETYLCHOLINE-RECEPTOR SYNTHESIS, IS A MEMBER OF THE NEU LIGAND FAMILY
FALLS, DL
ROSEN, KM
CORFAS, G
LANE, WS
FISCHBACH, GD
[J]. CELL, 1993, 72 (05) : 801 - 815
[7] HETERODIMERIZATION OF THE ERBB-1 AND ERBB-2 RECEPTORS IN HUMAN BREAST-CARCINOMA CELLS - A MECHANISM FOR RECEPTOR TRANSREGULATION
GOLDMAN, R
BENLEVY, R
PELES, E
YARDEN, Y
[J]. BIOCHEMISTRY, 1990, 29 (50) : 11024 - 11028
[8] PREVALENCE OF ABERRANT EXPRESSION OF THE EPIDERMAL GROWTH-FACTOR RECEPTOR IN HUMAN CANCERS
GULLICK, WJ
[J]. BRITISH MEDICAL BULLETIN, 1991, 47 (01) : 87 - 98
[9] A HEPARIN-BINDING GROWTH-FACTOR SECRETED BY MACROPHAGE-LIKE CELLS THAT IS RELATED TO EGF
HIGASHIYAMA, S
ABRAHAM, JA
MILLER, J
FIDDES, JC
KLAGSBRUN, M
[J]. SCIENCE, 1991, 251 (4996) : 936 - 939
[10] HUANG SS, 1992, J BIOL CHEM, V267, P11508

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