MICROCRYSTALS OF THE BETA(1) ISOENZYME OF PROTEIN-KINASE-C - AN ELECTRON-MICROSCOPIC STUDY

被引：11

作者：

NEWMAN, RH

CARPENTER, E

FREEMONT, PS

BLUNDELL, TL

PARKER, PJ

机构：

[1] IMPERIAL CANC RES FUND, PROT PHOSPHORYLAT LAB, LONDON WC2A 3PX, ENGLAND

[2] IMPERIAL CANC RES FUND, PROT STRUCT LAB, LONDON WC2A 3PX, ENGLAND

[3] UNIV LONDON BIRKBECK COLL, DEPT CRYSTALLOG, ICRF, STRUCT MOLEC BIOL UNIT, LONDON WC1E 7HX, ENGLAND

来源：

BIOCHEMICAL JOURNAL | 1994年 / 298卷

关键词：

D O I：

10.1042/bj2980391

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Microcrystals of protein kinase C beta(1) have been grown from solutions of poly(ethylene glycol). Image analysis of electron micrographs of the protein crystals, which diffracted to 5.0 nm, revealed p3 symmetry with a unit cell of about 10.3 nm x 10.3 nm. The electron stain-excluding densities showed a three-domain ring structure in projection, giving kidney bean-shaped molecules of about 7.0 nm x 4.5 nm diameter, packed as trimers. The implications of these observations for the function of the enzyme are discussed.

引用

页码：391 / 393

页数：3

共 12 条

[1]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[2] PROTEIN-KINASE-C CONTAINS A PSEUDOSUBSTRATE PROTOTYPE IN ITS REGULATORY DOMAIN [J].