YEAST GLOBAL TRANSCRIPTIONAL REGULATORS SIN4 AND RGR1 ARE COMPONENTS OF MEDIATOR COMPLEX RNA-POLYMERASE-II HOLOENZYME

被引：207

作者：

LI, Y

BJORKLUND, S

JIANG, YW

KIM, YJ

LANE, WS

STILLMAN, DJ

KORNBERG, RD

机构：

[1] STANFORD UNIV,SCH MED,DEPT BIOL STRUCT,STANFORD,CA 94305

[2] UNIV UTAH,HLTH SCI CTR,DEPT ONCOL SCI,DIV MOLEC BIOL & GENET,SALT LAKE CITY,UT 84132

[3] HARVARD MICROCHEM FACIL,CAMBRIDGE,MA 02138

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1995年 / 92卷 / 24期

关键词：

SACCHAROMYCES CEREVISIAE; REPRESSOR; GAL11;

D O I：

10.1073/pnas.92.24.10864

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Sin4 and Rgr1 proteins, previously shown by genetic studies to play both positive and negative roles in the transcriptional regulation of many genes, are identified here as components of mediator and RNA polymerase II holoenzyme complexes. Results with Sin4 deletion and Rgr1 truncation strains indicate the association of these proteins in a subcomplex comprising Sin4, Rgr1, Gal11, and a 50-kDa polypeptide. Taken together with the previous genetic evidence, our findings point to a role of the mediator in repression as well as in transcriptional activation.

引用

页码：10864 / 10868

页数：5

共 27 条

[1] TSF3, A GLOBAL REGULATORY PROTEIN THAT SILENCES TRANSCRIPTION OF YEAST GAL GENES, ALSO MEDIATES REPRESSION BY ALPHA-2 REPRESSOR AND IS IDENTICAL TO SIN4 [J].