LACK OF GLYCOSILATION OF PROOPIOMELANOCORTIN MIGHT ACCOUNT FOR THE PERIODIC ACID SCHIFF-NEGATIVE REACTION IN ACTH CELLS OF TELEOST FISHES

被引：8

作者：

ITURRIZA, FC ^{[1
]}

ESTIVARIZ, FE ^{[1
]}

机构：

[1] NATL UNIV LA PLATA, FAC CIENCIAS MED, CTR ESTUDIOS ENDOCRINOS, CASILLA CORREO 455, RA-1900 LA PLATA, ARGENTINA

来源：

GENERAL AND COMPARATIVE ENDOCRINOLOGY | 1986年 / 61卷 / 02期

关键词：

D O I：

10.1016/0016-6480(86)90200-5

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Unlike tetrapod ACTH cells, teleost ACTH cells do not react with the periodic acid-Schiff method (PAS). To find an explanation for this unique feature, chromatographic fractions obtained after filtration of pituitary extracts of Prochilodus platensis in Sephadex were immunologically analyzed. A high-molecular-weight protein which was identified as pro-opiomelanocortin (POMC) was detected. When this POMC was submitted to affinity chromatography in concanavalin binding, it was not detected. Furthermore, pituitaries incubated in media containing [3H]glucosamine or [3H]fucose did not incorporate these amino acids to the newly synthesized POMC. The results obtained strongly suggest an inability of the fish to glycosilate POMC, and this failure could account for the PAS-negative reaction in the ACTH cells.

引用

页码：229 / 236

页数：8

共 22 条

[1] IDENTIFICATION OF ACTH CELLS IN PITUITARY OF 2 TELEOSTS POECILIA LATIPINNA AND ANGUILLA ANGUILLA - CORRELATED CHANGES IN INTERRENAL AND IN PARS DISTALIS RESULTING FROM ADMINISTRATION OF METOPIRONE (SU 4885) [J].