C-HA-RAS MUTANTS WITH POINT MUTATIONS IN GLN-VAL-VAL REGION HAVE REDUCED INHIBITORY ACTIVITY TOWARD CATHEPSIN-B

被引：19

作者：

HIWASA, T ^{[1
]}

FUJITAYOSHIGAKI, J ^{[1
]}

SHIROUZU, M ^{[1
]}

KOIDE, H ^{[1
]}

SAWADA, T ^{[1
]}

SAKIYAMA, S ^{[1
]}

YOKOYAMA, S ^{[1
]}

机构：

[1] UNIV TOKYO,FAC SCI,DEPT BIOPHYS & BIOCHEM,BUNKYO KU,TOKYO 113,JAPAN

来源：

CANCER LETTERS | 1993年 / 69卷 / 03期

关键词：

C-HA-RAS; ONCOGENE PRODUCT; PROTEASE INHIBITOR; CATHEPSIN;

D O I：

10.1016/0304-3835(93)90169-A

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Protease-inhibitory activity of recombinant Ha-ras gene products (Ras) toward papain and cathepsins B and L was investigated. v-Ha-Ras showed more potent inhibitory activity toward cathepsin B as compared with c-Ha-Ras. We have also investigated protease-inhibitory activity of c-Ha-Ras mutants with point mutations in amino acids between positions 23 and 50. Inhibitory activity of Ras toward papain and cathepsin L was not largely altered among mutants. However, the inhibitory activity toward cathepsin B was significantly impaired by a mutation at position 43, 44, 45 or 48. These results suggest that 43Gln-Val-Val sequence plays an important role at least to inhibit cathepsin B.

引用

页码：161 / 165

页数：5

共 19 条

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