A PEPTIDE FROM THE CAUDAL NEUROSECRETORY-SYSTEM OF THE DOGFISH SCYLIORHINUS-CANICULA THAT IS STRUCTURALLY RELATED TO UROTENSIN-I

被引：17

作者：

WAUGH, D

ANDERSON, G

ARMOUR, KJ

BALMENT, RJ

HAZON, N

CONLON, JM

机构：

[1] CREIGHTON UNIV,SCH MED,CTR REGULATORY PEPTIDE,DEPT BIOMED SCI,OMAHA,NE 68178

[2] UNIV ST ANDREWS,GATTY MARINE LAB,ST ANDREWS KY16 8LB,FIFE,SCOTLAND

[3] UNIV MANCHESTER,SCH BIOL SCI,MANCHESTER M13 9PT,LANCS,ENGLAND

来源：

GENERAL AND COMPARATIVE ENDOCRINOLOGY | 1995年 / 99卷 / 03期

关键词：

D O I：

10.1006/gcen.1995.1117

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Using reversed-phase HPLC in combination with a radioimmunoassay for ovine corticotropin-releasing hormone (CRH), a peptide with CRH-like immunoreactivity was isolated in pure form from an extract of the caudal spinal cord region of the spotted dogfish, Scyliorhinus canicula. The primary structure of the peptide was established as Pro-Ala-Glu-Thr-Pro-Asn-Ser-Leu-Asp-Leu(10)-Thr-Phe-His-Leu-Leu-Arg-Glu-Met-Ile-Glu(20)-Ile-Ala-Lys-His-Glu-Asn-Gln-Gln-Met-Gln(30)-Ala-Asp-Ser-Asn-Arg-Arg-Ile-Met-Asp-Thr(40)-Ile . NH2. This amino acid sequence shows moderate structural similarity to Catostomus urotensin I (51%) and to human CRH (56%). The data provide, therefore, chemical evidence to support the conclusions of earlier immunohistochemical studies that the diffuse caudal neurosecretory system of elasmobranchs produces a peptide that is immunochemically related to teleost urotensin I peptides. However, the primary structure of urotensin I has been poorly conserved during evolution. (C) 1995 Academic Press, Inc.

引用

页码：333 / 339

页数：7

共 28 条

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