IDENTIFICATION OF BIOLOGICALLY-ACTIVE INHIBIN IN THE PERITONEAL-FLUID OF WOMEN

被引：5

作者：

BILLIAR, RB

HEMMINGS, R

SMITH, P

GROOME, N

机构：

[1] Department of Obstetrics and Gynecology, McGill University Faculty of Medicine, Montreal, Quebec

[2] School of Biological and Molecular Sciences, Oxford Brookes University Headington, Oxford

来源：

JOURNAL OF ASSISTED REPRODUCTION AND GENETICS | 1995年 / 12卷 / 01期

关键词：

PERITONEAL FLUID; DIMERIC INHIBIN AND ASSAYS; LATE FOLLICULAR PHASE;

D O I：

10.1007/BF02214130

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Purpose: Immunoreactive inhibin (i-inhibin) has been reported to be present in the peritoneal fluid of women. The radioimmunoassay employed measures free, biologically inactive alpha-subunits(s) equally as well as dimeric, biologically active inhibin. The present study was designed to determine if biologically active, dimeric inhibin is present in the peritoneal fluid of women. Methods: Peritoneal fluid of four women was assayed by radioimmunoassay, a sheep pituitary bioassay, and two ELISA procedures which utilized specific monoclonal antibodies for the ''capture'' of the alpha-subunit (ELISA-A) or the beta-subunit (ELISA-B) of inhibin and subsequent quantification of dimeric inhibin-A. Results: There was a good correlation between the values obtained by radioimmunoassay, bioassay, and bath ELISAs; two samples (from the late follicular phase) with relatively high i-inhibin concentrations were positive in all four assays, whereas two samples (from the early follicular phase) with very low i-inhibin concentrations were negative in the bioassay and ELISAs. Conclusion: A significant portion of the immunoreactive inhibin in the peritoneal fluid obtained during the late follicular phase of women is dimeric, biologically active inhibin. We speculate that this may have potential implications for oocyte maturation and early embryogenesis within the oviduct.

引用

页码：55 / 60

页数：6

共 26 条

[1]

Hemmings R., Falcone T., Billiar R.B., Tulandi T., Peritoneal fluid inhibin during the menstrual cycle, Obstet Gynecol, 80, pp. 27-29, (1992)

[2]

McLachlan R.I., Robertson D.M., Burger H.G., de Kretser D.M., The radioimmuno-assay of bovine and human follicular fluid and serum inhibin, Mol Cell Endocrinol, 46, pp. 175-185, (1986)

[3]

O W-S, Robertson D.M., de Kretser D.M., Inhibin as an oocyte meiotic inhibitor, Mol Cell Endocrinol, 62, pp. 307-311, (1989)

[4]

Phocas I., Sarandakou A., Rizos D., Dimitriadou F., Mantzavinos T., Zourlas P.A., Secretion of α-immunoreactive inhibin by human preembryos cultured in vitro, Hum Reprod, 7, pp. 545-549, (1992)

[5]

Roberts V.J., Sawchenko P.E., Vale W., Expression of inhibin/activin subunit messenger ribonucleic acids during rat embryogenesis, Endocrinology, 128, pp. 3122-3129, (1991)

[6]

Schneyer A.L., Mason A.J., Burton L.E., Ziegner J.R., Crowley W.F., Immuno-reactive inhibin α-subunit in human serum: implications for radioimmunoassay, J Clin Endocrinol Metab, 70, pp. 1208-1212, (1990)

[7]

Knight P.G., Beard A.J., Wrathall J.H.M., Castillo R.J., Evidence that the bovine ovary secretes large amounts of monomeric inhibin alpha subunit and its isolation from bovine follicular fluid, J Mol Endocrinol, 2, pp. 189-200, (1989)

[8]

Robertson D.M., Giacometti M., Foulds L.M., Lahnstein J., Goss N.H., Hearn M.T.W., deKretser D.M., solation of inhibin α-subunit precursor proteins from bovine follicular fluid, Endocrinology, 125, pp. 2141-2149, (1989)

[9]

Sugino K., Nakamura T., Takio K., Titani K., Miyamoto K., Hasegawa Y., Igarashi M., Sugino H., Inhibin alpha-subunit monomer is present in bovine follicular fluid, Biochem Biophys Res Commun, 159, pp. 1323-1329, (1989)

[10]

Miyamato K., Hasegawa Y., Fukuda M., Igarashi M., Demonstration of high molecular weight forms of inhibin in bovine follicular fluid (bFF) by using monoclonal antibodies to (bFF) 32K inhibin, Biochemical and Biophysical Research Communications, 136, pp. 1103-1109, (1986)

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