A CASSETTE VECTOR FOR PROTEIN ENGINEERING THE LANTIBIOTIC NISIN

被引：16

作者：

DODD, HM

HORN, N

GASSON, MJ

机构：

[1] Department of Genetics and Microbiology, Institute of Food Research, Norwich Research Park, Colney, Norwich

来源：

GENE | 1995年 / 162卷 / 01期

关键词：

LACTOCOCCUS; SHUTTLE PLASMID; MUTAGENESIS; ANTIMICROBIAL; DEHYDROALANINE; POSTTRANSLATIONAL MODIFICATION; COMPLEMENTATION;

D O I：

10.1016/0378-1119(95)00342-4

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Expression vectors are described that make use of a plasmid-encoded nisA cassette encoding the peptide component of nisin. Specific mutations can readily be incorporated throughout the coding region of nisA. Using this Vector in a nisA mutant host, three variant nisins, with dehydroalanine (Dha) residues changed to Ala residues, were generated.

引用

页码：163 / 164

页数：2

共 6 条

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[3] Engelke, Gutowski-Eckel, Kiesau, Seigers, Hammelmann, Entian, Regulation of nisin biosynthesis and immunity in Lactococcus lactis 6F3, Appl. Environ. Microbiol., 60, pp. 814-825, (1994)
[4] Jung, Lantibiotics: a survey, Nisin and Novel Lantibiotics, pp. 1-34, (1991)
[5] Kuipers, Beerthuyzen, Siezen, De Vos, Characterization of the nisin gene cluster nisABTCIPR of Lactococcus lactis, Eur. J. Biochem., 216, pp. 281-291, (1993)
[6] Van Rooijen, Gasson, De Vos, Characterization of the Lactococcus lactis lactose operon promoter: contribution of flanking sequences and LacR repressor to promoter activity, J. Bacteriol., 174, pp. 2273-2280, (1992)

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