TN5-INDUCED MUTATIONS AFFECTING SULFUR-OXIDIZING ABILITY (SOX) OF THIOSPHAERA-PANTOTROPHA

被引:41
作者
CHANDRA, TS [1 ]
FRIEDRICH, CG [1 ]
机构
[1] UNIV GOTTINGEN, INST MIKROBIOL, D-3400 GOTTINGEN, FED REP GER
关键词
D O I
10.1128/jb.166.2.446-452.1986
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Mutants of Thiosphaera pantotropha defective in chemolithoautotrophic growth were obtained by transpositional mutagenesis with Tn5 coding for kanamycin resistance. The suicide vehicle for introducing Tn5 to T. pantotropha was pSUP5011 harbored by Escherichia coli. Kanamycin-resistant isolates were screened for the inability to grow with reduced sulfur compounds (Sox-). Four classes of Sox- mutants were obtained. Three were of different pleiotropic phenotypes: (i) unable to grow with formate, nitrate, and xanthine; (this class strongly suggested the involvement of a molybdenum cofactor in inorganic sulfur-oxidizing ability); (ii) no growth with hydrogen; (iii) slight growth with hydrogen and formate. Two plasmids, pHG41 (about 450 kilobase pairs) and pHG42 (110 kilobases), were identified in lysates of T. pantotropha. In one Sox- mutant pHG41 could not be detected. Revertant analysis suggests that pHG41 and pHG42 were not involved in the Sox character.
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页码:446 / 452
页数:7
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