METHOD FOR HIGH-YIELD PREPARATION IN LARGE AND SMALL-SCALE OF NUCLEIC-ACIDS FROM MYCOBACTERIA

被引：23

作者：

SANTOS, AR

DEMIRANDA, AB

LIMA, LM

SUFFYS, PN

DEGRAVE, WM

机构：

[1] OSWALDO CRUZ FDN,DEPT TROP MED,LEP ROSY SECTOR,RIO DE JANEIRO,BRAZIL

[2] OSWALDO CRUZ FDN,DEPT BIOCHEM & MOLEC BIOL,RIO DE JANEIRO,BRAZIL

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 1992年 / 15卷 / 02期

关键词：

GENOMIC LIBRARY; MYCOBACTERIA; NUCLEIC ACID EXTRACTION;

D O I：

10.1016/0167-7012(92)90072-C

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A simple and very straightforward method for the extraction, in both large scale and micro-scale, of high molecular weight DNA and RNA from mycobacteria is described. The method requires few manipulations, is independent of previous mycobacterial culture conditions and is extremely efficient, yielding nucleic acids of very high quality which allowed the construction of genomic libraries of Mycobacterium leprae, M. tuberculosis and M. bovis BCG. The influence of several variables, such as mechanical treatment and chemicals used is discussed.

引用

页码：83 / 94

页数：12

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