PHOSPHATIDYLINOSITOL 3-KINASE ACTIVITY IN MURINE ERYTHROLEUKEMIA-CELLS DURING DMSO-INDUCED DIFFERENTIATION

被引:18
作者
AI, ZW
MISRA, S
SUSA, M
VARTICOVSKI, L
COHEN, CM
机构
[1] ST ELIZABETHS MED CTR,DEPT BIOMED RES,BOSTON,MA 02135
[2] TUFTS UNIV,SCH MED,DEPT PHYSIOL & MED,BOSTON,MA 02155
[3] TUFTS UNIV,SCH MED,DEPT ANAT & CELLULAR BIOL,BOSTON,MA 02155
关键词
D O I
10.1006/excr.1995.1252
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
We have used murine erythroleukemia cells (MEL cells) to investigate the role of phosphatidylinositol 3-kinase (PI 3-kinase) in erythroid differentiation. When treated with dimethyl sulfoxide (DMSO), MEL cells grown on a fibronectin matrix become committed to erythroid differentiation asynchronously, with 90% of cells becoming committed by Day 3 of treatment. We found that during the first 3 days of DMSO treatment MEL cells showed a twofold increase in total PI 3-kinase activity and a fourfold increase in the highly phosphorylated PI 3-kinase product, PIP3. At the same time there was no change in the content of p85, the PI 3-kinase regulatory subunit. After Day 3, PI 3-kinase activity declined, in parallel with a disappearance of p85 antigen from the cells. Inclusion of the PI 3-kinase inhibitor Wortmannin in the culture medium resulted in an inhibition of cellular PI 3-kinase activity and a delay in DMSO-induced erythroid differentiation. These data suggest that PI 3-kinase may play a critical role during commitment of MEL cells to erythroid differentiation. (C) 1995 Academic Press, Inc.
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页码:454 / 460
页数:7
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