THE SPECTRA OF BASE SUBSTITUTIONS INDUCED BY THE IMPCAB, MUCAB AND UMUDC ERROR-PRONE DNA-REPAIR OPERONS DIFFER FOLLOWING EXPOSURE TO METHYL METHANESULFONATE

被引:12
作者
DOYLE, N
STRIKE, P
机构
[1] Department of Genetics and Microbiology, Donnan Laboratories, University of Liverpool, Liverpool, L69 3BX
来源
MOLECULAR & GENERAL GENETICS | 1995年 / 247卷 / 06期
关键词
IMPCAB; MUCAB; UMUDC; ERROR-PRONE DNA REPAIR; MUTAGENIC SPECTRA;
D O I
10.1007/BF00290405
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We have used the lacZ reversion assay to study the mutation spectra induced by the Escherichia coli chromosomal umuDC operon and of its two plasmid-borne analogues impCAB and mucAB following exposure of cells to UV light and methyl methanesulfonate (MMS). We have shown that the impCAB, mucAB and umuDC operons all produce a similar response to UV light which results almost exclusively in AT --> GC transitions. However, we found that the three operons produced different responses to alkylating agents. We found that with MMS the chromosomal umuDC operon produced almost exclusively AT --> GC transitions, whilst both mucAB and impCAB produced predominantly transversions. In the case of the impCAB operon the mutation spectrum contained more AT --> TA than GC --> TA transversions; this balance was reversed with mucAB. The effect of the copy number of the error-prone DNA repair operons upon the mutagenic spectra was also studied. The results obtained suggest that the copy number of the imp operon does not greatly affect the specificity of base substitutions observed. However, an increase in the copy number of the umuDC operon greatly affected the specificity of base substitution, such that virtually no transitions were produced and the spectrum was dominated by GC/AT --> TA transversions. It appears that the three error-prone DNA repair operons impCAB, mucAB and umuDC, despite showing strong structural and functional homologies, can display major differences in the spectrum of base changes induced during mutagenesis. We propose that the type of misincorporation/chain extension which DNA polymerase III is allowed to synthesize on a damaged DNA template is extremely sensitive to both the amount and type of error-prone repair proteins present. The modulation of these events by the different proteins can result in widely different mutagenic changes in the repaired DNA.
引用
收藏
页码:735 / 741
页数:7
相关论文
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