MAS NMR STRUCTURE REFINEMENT OF UNIFORMLY C-13 ENRICHED CHLOROPHYLL-A WATER AGGREGATES WITH 2D DIPOLAR CORRELATION SPECTROSCOPY

被引：49

作者：

BOENDER, GJ ^{[1
]}

RAAP, J ^{[1
]}

PRYTULLA, S ^{[1
]}

OSCHKINAT, H ^{[1
]}

DEGROOT, HJM ^{[1
]}

机构：

[1] EUROPEAN MOLEC BIOL LAB,D-69117 HEIDELBERG,GERMANY

来源：

CHEMICAL PHYSICS LETTERS | 1995年 / 237卷 / 5-6期

关键词：

D O I：

10.1016/0009-2614(95)00357-A

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

2D MAS dipolar correlation spectroscopy of uniformly C-13 enriched chlorophyll a/water aggregates was performed using broadband RFDR to promote exchange of coherence through homonuclear dipolar couplings. It is shown experimentally that incorporation of TPPI and coherence pathway selection in the RFDR pulse scheme yields virtually pure 2D absorption lineshapes. From a series of 2D correlation spectra collected at two spinning speeds with different mixing times of similar to 1 and similar to 10 ms all C-13 resonances of the chlorophyll a molecule were assigned. The spectra with the longer mixing times reveal through-space intermolecular polarization transfer. This demonstrates that structural information can be obtained from uniformly C-13 enriched samples, which paves the way for a full structural characterization of amorphous solids.

引用

页码：502 / 508

页数：7

共 22 条

[1]

Abraham R. J., 1991, CHLOROPHYLLS, P797

[2] CHEMICAL-SHIFT CORRELATION SPECTROSCOPY IN ROTATING SOLIDS - RADIO FREQUENCY-DRIVEN DIPOLAR RECOUPLING AND LONGITUDINAL EXCHANGE [J].