PURIFICATION OF HOMOLOGOUS PROTEIN CARBOXYL METHYLTRANSFERASE ISOZYMES FROM HUMAN AND BOVINE ERYTHROCYTES

被引:56
作者
GILBERT, JM
FOWLER, A
BLEIBAUM, J
CLARKE, S
机构
[1] UNIV CALIF LOS ANGELES, DEPT CHEM & BIOCHEM, LOS ANGELES, CA 90024 USA
[2] UNIV CALIF LOS ANGELES, DEPT BIOL CHEM, LOS ANGELES, CA 90024 USA
[3] UNIV CALIF LOS ANGELES, INST MOLEC BIOL, LOS ANGELES, CA 90024 USA
关键词
D O I
10.1021/bi00414a042
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
we have purified the two major isozymes of the L-isoaspartyl/D-aspartyl protein methyltransferase from both human and bovine erythrocytes. These four enzymes all have polypeptide molecular weights of approximately 26,500 and appear to be monomers in solution. Each of these enzymes cross-reacts with antibodies directed against protein carboxyl methyltransferase I from bovine brain. Their structures also appear to be similar when analyzed by dodecyl sulfate gel electrophoresis for the large fragments produced by digestion with Staphylococcus aureus protease V8 or when analyzed by high-performance liquid chromatography (HPLC) for tryptic peptides. The structural relatedness of these enzymes was confirmed by sequence analysis of a total of 433 residues in 32 tryptic fragments of the human erythrocytes isozymes I and II and of bovine erythrocyte isozyme II. We found sequence identity or probable identity in 111 out of 112 residues when we compared the human isozymes I and II and identities in 127 out of 134 residues when the human and bovine isozymes II were compared. These results suggest that the erythrocyte isozymes from both organisms may have nearly identical structures and confirm the similarities in the function of these methyltransferases that have been previously demonstrated.
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页码:5227 / 5233
页数:7
相关论文
共 30 条
[1]  
ASWAD DW, 1984, J BIOL CHEM, V259, P714
[2]   PURIFICATION AND CHARACTERIZATION OF 2 DISTINCT ISOZYMES OF PROTEIN CARBOXYMETHYLASE FROM BOVINE BRAIN [J].
ASWAD, DW ;
DEIGHT, EA .
JOURNAL OF NEUROCHEMISTRY, 1983, 40 (06) :1718-1726
[3]  
BENAVRAM CM, 1986, P NATL ACAD SCI USA, V83, P4183
[4]   RAPID ANALYSIS OF AMINO-ACIDS USING PRE-COLUMN DERIVATIZATION [J].
BIDLINGMEYER, BA ;
COHEN, SA ;
TARVIN, TL .
JOURNAL OF CHROMATOGRAPHY, 1984, 336 (01) :93-104
[5]  
BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[6]  
BURNETTE WN, 1981, ANAL BIOCHEM, V112, P195, DOI 10.1016/0003-2697(81)90281-5
[7]  
CLARKE S, 1985, ANNU REV BIOCHEM, V54, P479, DOI 10.1146/annurev.biochem.54.1.479
[8]  
CLEVELAND DW, 1977, J BIOL CHEM, V252, P1102
[9]  
Dayhoff M., 1978, Atlas Protein Seq. Struct, V5, P345352
[10]  
Dickerson R.E., 1983, Hemoglobin: Structure, Function, Evolution, and Pathology